… To generate active-state-specific nanobody clones, we performed two rounds of MACS to
generate a library of A 2A R-binding nanobodies followed by one round of FACS to enrich …

G-protein-coupled receptors (GPCRs) play critical roles in regulating physiological processes
ranging from neurotransmission to cardiovascular function. Current methods for tracking …

How the cell converts graded signals into threshold‐activated responses is a question of
great biological relevance. Here, we uncover a nonlinear modality of epidermal growth factor …

The immune checkpoint receptor PD-1 and its ligand, PD-L1, have emerged as key regulators
of anti-tumor immunity in humans. Recently, we reported an ultra-high-affinity PD-1 mutant…

… Pascolutti et al. describe an additional layer of regulation of EGFR signaling, relying on
the sequestration of receptors into molecularly distinct clathrin-coated vesicles that regulate …

Rab-interacting lysosomal protein (RILP) is a downstream effector of the Rab7 GTPase. GTP-bound
Rab7 recruits RILP to endosomal membranes and, together, they control late …

Adiponectin is a highly abundant protein hormone secreted by adipose tissue. It elicits
diverse biological responses, including anti-diabetic, anti-inflammatory, anti-tumor, and anti-…

In many cell types, septins assemble into filaments and rings at the neck of cellular appendages
and/or at the cleavage furrow to help compartmentalize the plasma membrane and …

Simple Summary Peptide-based cancer vaccines require adjuvants to increase their
immunogenicity to mount an antigen-specific reliable immune response. We have previously shown …

Camelid single-domain antibody fragments (“nanobodies”) provide the remarkable specificity
of antibodies within a single immunoglobulin V HH domain. This unique feature enables …