Across the eukaryotic phylogeny, offspring usually inherit their mitochondrial genome from
only one of two parents: in animals, the female. Although mechanisms that eliminate …

10.7554/eLife.08469.001 Previously, we described a large collection of Minos-Mediated
Integration Cassettes (MiMICs) that contain two phiC31 recombinase target sites and allow the …

High copy number and random segregation confound genetic analysis of the mitochondrial
genome. We developed an efficient selection for heritable mitochondrial genome (mtDNA) …

Cse4p is an essential histone H3 variant in Saccharomyces cerevisiae that defines
centromere identity and is required for proper segregation of chromosomes. In this study, we …

Using the yeast GAL4 transcription factor to control expression in Drosophila melanogaster
has long been ineffective in female germ cells during oogenesis. Here, DeLuca and …

Polycomb silencing represses gene expression and provides a molecular memory of chromatin
state that is essential for animal development. We show that Drosophila female germline …

… 0 exonuclease and replication per se are not involved in elimination, we show a tight coupling
between association of a large Tam-containing particle with nucleoids of decreasing size. …

… After tobacco etch virus protease cleavage to remove the His6-tag, the protein was further
purified using cation exchange and size exclusion chromatography. APC, Cdc20, and securin (…

… Remarkably, the size of such filaments increased dramatically during oogenesis, forming
an extensive three-dimensional network of Corolla-positive filaments within the nucleus of …

A simple measurement, the average interpleural distance (AID), which is obtained from
three linear measurements, closely predicts true pneumothorax size as determined by …