… The 28S, 18S, and 5.8S rRNAs are assembled with ribosomal proteins (RPs) to form the
small and large preribosome subunits, which are each exported separately to the cytoplasm …

The identification of interaction partners in protein complexes is a major goal in cell biology.
Here we present a reliable affinity purification strategy to identify specific interactors that …

… HSP90 and hSpagh are also involved in the production of snoRNPs (Boulon et al., 2008),
and … S. Boulon is a long-term fellow of the Human Frontier Science Program (HFSP), and DM …

… To verify these results, we performed GST pull-down experiments with in vitro–translated
35 S-labeled proteins. In agreement with the Y2H results, we found that Nufip directly bound …

To better understand intranuclear-targeting mechanisms, we have studied the transport of
U3 snoRNA in human cells. Surprisingly, we found that PHAX, the snRNA export adaptor, is …

… with in vitro-translated 35 S-labeled 15.5K, fibrillarin, NOP56, … S-labeled box C/D core
proteins (Fig. 2B , lane 3). GST, which was used as a negative control, did not retain any of the 35 S…

… 1B), and therefore dictates control(s) required to accurately distinguish changes due to
biologically relevant effects. An initial, exploratory IP experiment is usually recommended. Tricks …

The morphology and composition of subnuclear organelles, such as Cajal bodies (CBs),
nucleoli, and other nuclear bodies, is dynamic and can change in response to a variety of cell …

… 10 min, pellets were extracted a second time 4C in 50 mM Tris HCl, pH 7.5, 150 mM NaCl,
1% NP-40, 0.5% deoxycholate, and Complete antiprotease cocktail and sonicated for 3 × 10 s…

… Extracts were briefly sonicated on ice (3 × 10 s at full power) and then cleared by centrifuging
at 2,800 × g (3,500 rpm; GH3.8 rotor, Beckman Coulter GS-6) for 10 min at 4 C, and total …