Conspectus Over just the last 2 years, mRNA therapeutics and vaccines have undergone a
rapid transition from an intriguing concept to real-world impact. However, whereas some …

… We set the 533 nm LED to 0.40 μmol m −2 s −1 and each bottom LED to 3.21 μmol m −2 s
−1 because CcaS-CcaR has approximately 10-fold greater sensitivity to green than red light (…

… coli and S. cerevisiae gene expression systems and two different flow cytometers. First, … ’s
compatibility with eukaryotic cells by calibrating Venus measurements from a light-inducible S. …

… Total LED power output in μmol s −1 was measured using a spectrometer (StellarNet UVN-…
of 20 μmol m −2 s −1 or more for red LEDs and 50 μmol m −2 s −1 or more for green LEDs. …

… An activating interaction catalyzes the conversion of an enzyme’s inactive form into the active
form. For example, an activating interaction of component A over component B, denoted by …

… A one-sample Student’s t test was conducted for every sample to evaluate whether sfGFP
fluorescence was significantly different from zero (one-sided, p < 0.05). Fluorescence of …

… This study demonstrates that the S. cerevisiae TF Msn2 translocates to the nucleus with
different dynamics depending on the nature and magnitude of the stress. 1-NM-PP1 is also used …

… Diagrams are found at every stage of a system’s development, from idea and requirements
capture to refinement and realization, from analysis and debugging to deployment, operations…

… predictive accuracy m of a model, the precision μ of property value measurements, the
estimated range r̂ over which a property value will fulfill its specification, and the probability p s …

mRNA therapeutics are revolutionizing the pharmaceutical industry, but methods to optimize
the primary sequence for increased expression are still lacking. Here, we design 5'UTRs for …