User profiles for S. Darmanis

Spyros Darmanis

Genentech
Verified email at gene.com
Cited by 9129

Single-cell proteomics enabled by next-generation sequencing or mass spectrometry

HM Bennett, W Stephenson, CM Rose, S Darmanis - Nature Methods, 2023 - nature.com
In the last decade, single-cell RNA sequencing routinely performed on large numbers of single
cells has greatly advanced our understanding of the underlying heterogeneity of complex …

A survey of human brain transcriptome diversity at the single cell level

S Darmanis, SA Sloan, Y Zhang… - Proceedings of the …, 2015 - National Acad Sciences
The human brain is a tissue of vast complexity in terms of the cell types it comprises. Conventional
approaches to classifying cell types in the human brain at single cell resolution have …

[HTML][HTML] Single-cell transcriptomics of 20 mouse organs creates a Tabula Muris: The Tabula Muris Consortium

…, S Darmanis, J Batson, O Botvinnik, MB Chen, S Chen… - Nature, 2018 - ncbi.nlm.nih.gov
We have created a compendium of single cell transcriptomic data from the model organism
Mus musculus comprising more than 100,000 cells from 20 organs and tissues. These data …

[PDF][PDF] Single-cell RNA-seq analysis of infiltrating neoplastic cells at the migrating front of human glioblastoma

S Darmanis, SA Sloan, D Croote, M Mignardi… - Cell reports, 2017 - cell.com
… Our analysis revealed cellular variation in the tumor’s genome and transcriptome. We
were also able to identify infiltrating neoplastic cells in regions peripheral to the core lesions. …

[PDF][PDF] Developmental heterogeneity of microglia and brain myeloid cells revealed by deep single-cell RNA sequencing

Q Li, Z Cheng, L Zhou, S Darmanis, NF Neff, J Okamoto… - Neuron, 2019 - cell.com
… score and an S score, based on its expression of G2/M and S phase markers. Then for each
gene, the relationship between the gene expression and the G2M and S scores across all …

Ageing hallmarks exhibit organ-specific temporal signatures

…, AO Pisco, J Karkanias, NF Neff, A Keller, S Darmanis… - Nature, 2020 - nature.com
… ’s rank correlation coefficient was computed on the basis of expression and age. Similarly,
we computed Spearman’s rank … . In addition to Spearman’s rank correlation coefficients for …

[PDF][PDF] Human astrocyte maturation captured in 3D cerebral cortical spheroids derived from pluripotent stem cells

SA Sloan, S Darmanis, N Huber, TA Khan, F Birey… - Neuron, 2017 - cell.com
There is significant need to develop physiologically relevant models for investigating human
astrocytes in health and disease. Here, we present an approach for generating astrocyte …

[PDF][PDF] Therapy-induced evolution of human lung cancer revealed by single-cell RNA sequencing

…, RC Doebele, J Weissman, CM Blakely, S Darmanis… - Cell, 2020 - cell.com
… Many of the mutations we identified had not been previously reported by the clinical-grade
assay conducted on a patient’s tumor despite having been included in the clinical panel (…

[PDF][PDF] Simultaneous multiplexed measurement of RNA and proteins in single cells

S Darmanis, CJ Gallant, VD Marinescu, M Niklasson… - Cell reports, 2016 - cell.com
… ’s IFC HX according to the manufacturer’s instructions and then run in Fluidigm’s Biomark …
mix (50 C, 120 s; 70 C, 1,800 s; 25 C, 600 s), hot start (95 C, 300 s), and PCR cycling for 40 …

Corona mortis: an anatomical study with clinical implications in approaches to the pelvis and acetabulum

S Darmanis, A Lewis, A Mansoor… - Clinical Anatomy: The …, 2007 - Wiley Online Library
The “corona mortis” is an anatomical variant, an anastomosis between the obturator and the
external iliac or inferior epigastric arteries or veins. It is located behind the superior pubic …