Despite the importance of epigenetic regulation in neurological disorders, little is known
about neuronal chromatin. Cerebellar Purkinje neurons have large and euchromatic nuclei, …

The high level of 5-hydroxymethylcytosine (5hmC) present in neuronal genomes suggests
that mechanisms interpreting 5hmC in the CNS may differ from those present in embryonic …

… (A) Frameshift mutations are nucleotide(s) insertions or deletions that lead to loss of the open
reading frame. The protein amino acid sequence is different from the point of mutation and …

… Total RNA from tissue culture cells was purified using TRI‐Reagent (Sigma) according to
the manufacturer’s recommendations. Prior to cDNA synthesis RNA was treated with RQ1 …

… For pulldown with GST-MeCP2 fusion protein, [ 35 S]-labeled ATRX was incubated with …
For pulldown with the GST-ATRX fusion, [ 35 S]-labeled MeCP2 or its mutant derivatives …

Rett syndrome (RTT) is a severe neurological disorder caused by mutations in the X-linked
MECP2 gene, which encodes a methyl-CpG binding transcriptional repressor. Using the …

… Amplifications consisted of 35 cycles, (94C for 45 s, 49C for 60 s and 72C for 45 s; different
annealing temperatures were used for primers m1_0_for/m1_0_rev (55C), Fkpd/Fkpr (60C), …

Isocitrate dehydrogenase 1 mutations drive human gliomagenesis, probably through
neomorphic enzyme activity that produces D-2-hydroxyglutarate. To model this disease, we …

… in Nathaniel Heintz’s laboratory when Skirmantas Kriaucionis was elucidating the chromatin
… Kornberg’s classic experiments of 1961 and used in Adrian Bird’s pioneering experiments …

… As a third measure of DNA replication, we compared regions replicating early during S
phase to regions replicating late [11]. We calculated strand-specific signatures [15] that add …