User profiles for S. Lequime
Sebastian LequimeAssistant Professor, University of Groningen Verified email at rug.nl Cited by 1438 |
[HTML][HTML] Determinants of arbovirus vertical transmission in mosquitoes
Vertical transmission (VT) and horizontal transmission (HT) of pathogens refer to parental
and non-parental chains of host-to-host transmission. Combining HT with VT enlarges …
and non-parental chains of host-to-host transmission. Combining HT with VT enlarges …
Vertical transmission of arboviruses in mosquitoes: a historical perspective
S Lequime, L Lambrechts - Infection, Genetics and Evolution, 2014 - Elsevier
Arthropod-borne viruses (arboviruses) are mainly transmitted horizontally among vertebrate
hosts by blood-feeding invertebrate vectors, but can also be transmitted vertically in the …
hosts by blood-feeding invertebrate vectors, but can also be transmitted vertically in the …
Measles virus and rinderpest virus divergence dated to the sixth century BCE
… On the basis of these considerations, our substantially older MeV-RPV divergence estimate
provides grounds for sketching a new model of MeV’s evolutionary history. Under this …
provides grounds for sketching a new model of MeV’s evolutionary history. Under this …
[HTML][HTML] Recent African strains of Zika virus display higher transmissibility and fetal pathogenicity than Asian strains
… The thermocycling program consisted of 2 min at 95 C, 35 cycles of 30 s at 95 C, 30 s at 60
C, and 30 s at 72 C with a final extension step of 7 min at 72 C. Amplicons were visualized by …
C, and 30 s at 72 C with a final extension step of 7 min at 72 C. Amplicons were visualized by …
[PDF][PDF] Non-retroviral endogenous viral element limits cognate virus replication in Aedes aegypti ovaries
…, AB Crist, SH Merkling, A Fontaine, S Lequime… - Current Biology, 2020 - cell.com
… The lysate was centrifuged 15-30 s at 21,100g and incubated at room temperature (20-25
C) for at least 20 min. Subsequently, 0.5-1 μL of lysate was used directly into a 20-μl PCR …
C) for at least 20 min. Subsequently, 0.5-1 μL of lysate was used directly into a 20-μl PCR …
[HTML][HTML] Archival influenza virus genomes from Europe reveal genomic variability during the 1918 pandemic
… To identify the subunit(s) responsible for this difference, we determined the effect of swapping
single polymerase subunits between BM and MU-162 (Fig. 2b). The introduction of PA …
single polymerase subunits between BM and MU-162 (Fig. 2b). The introduction of PA …
[HTML][HTML] Genetic drift, purifying selection and vector genotype shape dengue virus intra-host genetic diversity in mosquitoes
S Lequime, A Fontaine, M Ar Gouilh… - PLoS …, 2016 - journals.plos.org
… S d is the number of synonymous substitutions in the sequence, S s is the number of
synonymous sites, N d is the number of non-synonymous substitutions in the sequence and N s is …
synonymous sites, N d is the number of non-synonymous substitutions in the sequence and N s is …
Cell-Fusing Agent Virus Reduces Arbovirus Dissemination in Aedes aegypti Mosquitoes In Vivo
… ) following the manufacturer’s guidelines and a reverse primer … to the manufacturer’s
instructions with primers located at … ) according to the manufacturer’s instructions to produce large …
instructions with primers located at … ) according to the manufacturer’s instructions to produce large …
[HTML][HTML] Epidemiological hypothesis testing using a phylogeographic and phylodynamic framework
Computational analyses of pathogen genomes are increasingly used to unravel the
dispersal history and transmission dynamics of epidemics. Here, we show how to go beyond …
dispersal history and transmission dynamics of epidemics. Here, we show how to go beyond …
[HTML][HTML] Excretion of dengue virus RNA by Aedes aegypti allows non-destructive monitoring of viral dissemination in individual mosquitoes
A Fontaine, D Jiolle, I Moltini-Conclois, S Lequime… - Scientific reports, 2016 - nature.com
… The images or other third party material in this article are included in the article’s Creative
Commons license, unless indicated otherwise in the credit line; if the material is not included …
Commons license, unless indicated otherwise in the credit line; if the material is not included …