… and qualifying as a medical doctor, Nakane decided to enter a four-year … Over the last two
years, Nakane has been developing … Nakane also contributes to several open-source projects, …

10.7554/eLife.42166.001 Here, we describe the third major release of RELION. CPU-based
vector acceleration has been added in addition to GPU support, which provides flexibility in …

The three-dimensional positions of atoms in protein molecules define their structure and their
roles in biological processes. The more precisely atomic coordinates are determined, the …

… T) or nsp3 (Abcam, ab181620; diluted 1:500 in PBS-T). Next, the membranes were washed
3 times in PBS-T… 1 h, washed again 3 times in PBS-T, incubated with western Lightning Plus-…

… the right shows the value of the supervised threshold (t = T). … the right shows the value of the
supervised threshold (t = T). … on the right shows the value of the supervised threshold (t = T). …

… -I, Continuum) to the sample at t = 0 ms and t = 10 ms (that is, the two flashes were separated
by 10 ms) for the pre-flashed samples, and t = 0 ms and t = 5 ms (separated by 5 ms) for the …

… can explain the requirement of the third T in the 50-TTTN-30 … with the requirement of the
second T in the PAM. The 5-… consistent with the requirement of the first T in the PAM. The K548A …

Deep medullary veins drain into subependymal veins with four convergence zones and show
parallel distribution patterns adjacent to the body or inferior horn and a radial pattern in the …

Bacteriorhodopsin (bR) is a light-driven proton pump and a model membrane transport protein.
We used time-resolved serial femtosecond crystallography at an x-ray free electron laser …

A new method to estimate the trajectories of particle motion and the amount of cumulative
beam damage in electron cryo-microscopy (cryo-EM) single-particle analysis is presented. The …