Bioelectrical understanding and engineering of cell biology

…, P Rocha, MBA Djamgoz, T Pilizota… - Journal of the …, 2020 - royalsocietypublishing.org
The last five decades of molecular and systems biology research have provided unprecedented
insights into the molecular and genetic basis of many cellular processes. Despite these …

[HTML][HTML] General calibration of microbial growth in microplate readers

…, AF McVey, IBN Clark, PS Swain, T Pilizota - Scientific reports, 2016 - nature.com
… We thank Jerko Rosko, Dejan Kunovac and all other members of the Pilizota and Swain lab
for their comments and support, Andrew Schofield for granting us access to the refractometer …

Conformational spread as a mechanism for cooperativity in the bacterial flagellar switch

F Bai, RW Branch, DV Nicolau Jr, T Pilizota, BC Steel… - science, 2010 - science.org
The bacterial flagellar switch that controls the direction of flagellar rotation during chemotaxis
has a highly cooperative response. This has previously been understood in terms of the …

Escherichia coli as a model active colloid: A practical introduction

…, A Dawson, T Vissers, D Miroli, T Pilizota… - Colloids and Surfaces B …, 2016 - Elsevier
… (5) ℓ g ( coli ) = k B T Δ ρ g V cell ≈ 4 μ m , for an E. coli with V cell … 4, give essentially
identical results, 9 so that the decreasing v ( t ) is not due to gradual kinematic accumulation at …

[HTML][HTML] Painting with light-powered bacteria

J Arlt, VA Martinez, A Dawson, T Pilizota… - Nature …, 2018 - nature.com
… (t), in illuminated squares of different sizes is shown in Fig. 3a, from which we extracted t D ,
… Equations (1) and (2) are also confirmed by the data collapse achieved for ρ tot (t) when we …

[HTML][HTML] Inferring time derivatives including cell growth rates using Gaussian processes

…, IBN Clark, J Vogel, T Pilizota - Nature …, 2016 - nature.com
… where we denote the growth curve as y(t). The doubling time is ln(2) times the inverse of the
growth rate. We define the lag time as the … If this point of maximum growth rate is at t=t*, then …

[HTML][HTML] Fast, Multiphase Volume Adaptation to Hyperosmotic Shock by Escherichia coli

T Pilizota, JW Shaevitz - PloS one, 2012 - journals.plos.org
… time of hyperosmotic shock, t 1 . For this, d(V(t))/dt was calculated using V(t) from the beginning
of … Then t 1 = t min−1 where t min is the time at which d(V(t))/dt is minimal and V min is the …

[HTML][HTML] Nonequivalence of membrane voltage and ion-gradient as driving forces for the bacterial flagellar motor at low load

CJ Lo, MC Leake, T Pilizota, RM Berry - Biophysical journal, 2007 - cell.com
… F T includes fluorescence due to dye bound to the membrane (F m ) as well as fluorescence
… Lines are exponential fits, F T = A 0 + A 1 exp{−t/t 0 }, time constants t 0 are given in the text. …

[PDF][PDF] Plasmolysis and cell shape depend on solute outer-membrane permeability during hyperosmotic shock in E. coli

T Pilizota, JW Shaevitz - Biophysical journal, 2013 - cell.com
… (A) Averaged total and cytoplasmic volume, (B) length, and (C) width traces in time during
dextran shocks of different magnitudes (Vn(t), Ln(t), Wn(t)). Data points are recorded at 0.5 Hz …

A molecular brake, not a clutch, stops the Rhodobacter sphaeroides flagellar motor

T Pilizota, MT Brown, MC Leake… - Proceedings of the …, 2009 - National Acad Sciences
Many bacterial species swim by employing ion-driven molecular motors that power the rotation
of helical filaments. Signals are transmitted to the motor from the external environment via …