… of the Tjian and Darzacq laboratory for their reading, insights, and helpful discussions on
this review. We would expressly like to thank the Tjian and Darzacq COVID-19 response team …

… The x-axis is the number of proteins from the 33 studies that were claimed to display that
evidence. (B) A simulated example of how diffraction-limited fluorescence imaging can obscure …

… Darzacq, the members of the Tjian-Darzacq laboratory, and the external reviewers, all of
whom we thank for their time and contributions. This work was supported by National Institutes …

… A 30 beam square lattice light sheet, with inner and outer numerical apertures of 0.505
and 0.60 respectively, was generated with a 488 nm input laser and dithered in x over a 5 μm …

… Goodrich, GS Martin, and members of Tjian and Darzacq labs for critical reading of the
manuscript. Funding: This work was supported by California Institute of Regenerative Medicine …

We imaged transcription in living cells using a locus-specific reporter system, which allowed
precise, single-cell kinetic measurements of promoter binding, initiation and elongation. …

Cajal (coiled) bodies are conserved subnuclear organelles that are present in the nucleoplasm
of both animal and plant cells. Although Cajal bodies were first described nearly 100 …

Transcription is reported to be spatially compartmentalized in nuclear transcription factories
with clusters of RNA polymerase II (Pol II). However, little is known about when these foci …

Understanding gene expression requires the ability to follow the fate of individual molecules.
Here we use a cellular system for monitoring messenger RNA (mRNA)expression to …

… frame and tracking them between frames, we obtaining several estimates of the true x,y
coordinates for each molecule, which improves the localization precision. Moreover, since each …