Sequence and annotation of 42 cannabis genomes reveals extensive copy number variation in cannabinoid synthesis and pathogen resistance genes

KJ McKernan, Y Helbert, LT Kane, H Ebling, L Zhang… - BioRxiv, 2020 - biorxiv.org
… To avoid interpreting genome-specific SVs as Y associated, we considered only CNVs
that exist in all males and females for X and Y categorization. Genes of interest on the Y

[HTML][HTML] Cannabis microbiome sequencing reveals several mycotoxic fungi native to dispensary grade Cannabis flowers

…, J Spangler, L Zhang, V Tadigotla, Y Helbert… - …, 2015 - ncbi.nlm.nih.gov
The Center for Disease Control estimates 128,000 people in the US are hospitalized annually
due to food borne illnesses. This has created a demand for food safety testing targeting …

[PDF][PDF] Sequencing of bivalent Moderna and Pfizer mRNA vaccines reveals nanogram to microgram quantities of expression vector dsDNA per dose

K McKernan, Y Helbert, LT Kane, S McLaughlin - OSFPreprints, 2023 - files.osf.io
Several methods were deployed to assess the nucleic acid composition of four vials of the
Moderna and Pfizer bivalent mRNA vaccines. Two vials from each vendor were evaluated …

[HTML][HTML] Metagenomic analysis of medicinal Cannabis samples; pathogenic bacteria, toxigenic fungi, and beneficial microbes grow in culture-based yeast and mold …

K McKernan, J Spangler, Y Helbert, RC Lynch… - …, 2016 - ncbi.nlm.nih.gov
Background: The presence of bacteria and fungi in medicinal or recreational Cannabis poses
a potential threat to consumers if those microbes include pathogenic or toxigenic species. …

[HTML][HTML] Single molecule sequencing of THCA synthase reveals copy number variation in modern drug-type Cannabis sativa L

KJ McKernan, Y Helbert, V Tadigotla, S McLaughlin… - BioRxiv, 2015 - biorxiv.org
… On the Y-axis is Heterozygosity where homozygous genotypes are either 1 or 0 and
heterozygous genotypes would display at 0.5. Samples Blueberry essence has been fully sampled …

[HTML][HTML] A whole genome atlas of 81 Psilocybe genomes as a resource for psilocybin production.

K McKernan, L Kane, Y Helbert, L Zhang… - …, 2021 - f1000research.com
… Sequence coverage of all contigs larger than 1 kb where coverage is on the Log Y axis
and contigs sorted by length (largest to smallest: left to right) are on the X axis. (Middle Right) …

[HTML][HTML] Whole genome sequencing of colonies derived from cannabis flowers and the impact of media selection on benchmarking total yeast and mold detection tools

K McKernan, Y Helbert, L Kane, N Houde… - …, 2021 - ncbi.nlm.nih.gov
Background: Cannabis products are subjected to microbial testing for human pathogenic
fungi and bacteria. These testing requirements often rely on non-specific colony forming unit (…

Cryptocurrencies and zero mode wave guides: an unclouded path to a more contiguous Cannabis sativa L. genome assembly.

K McKernan, Y Helbert, LT Kane, H Ebling, L Zhang… - 2018 - osf.io
We describe the use of a Decentralized Autonomous Organization (DAO) to crypto-fund the
single molecule sequencing and publication of a Type II Cannabis plant. This resulted in the …

DREAMing of a patent-free human genome for clinical sequencing

KJ McKernan, J Spangler, Y Helbert, L Zhang… - Nature …, 2013 - nature.com
… Yvonne Helbert … McKernan, Jessica Spangler, Yvonne Helbert, Lei Zhang and Vasisht
Tadigotla are at Courtagen Life Sciences, Woburn, Massachusetts, USA., … Yvonne Helbert

Microbiological examination of nonsterile Cannabis products

K McKernan, Y Helbert, H Ebling, A Cox, LT Kane… - 2018 - osf.io
Cannabis microbial testing presents unique challenges. Unlike food testing, cannabis testing
has to consider various routes of administration beyond just oral administration. Cannabis …