User profiles for B. J. Karas
 | Bogumil KarasAssistant Professor @ University of Western Ontario and CEO at Designer Microbes Inc., Verified email at uwo.ca Cited by 4119 |
Design and synthesis of a minimal bacterial genome
INTRODUCTION In 1984, the simplest cells capable of autonomous growth, the mycoplasmas,
were proposed as models for understanding the basic principles of life. In 1995, we …
were proposed as models for understanding the basic principles of life. In 1995, we …
A Cytokinin Perception Mutant Colonized by Rhizobium in the Absence of Nodule Organogenesis
In legumes, Nod-factor signaling by rhizobia initiates the development of the nitrogen-fixing
nodule symbiosis, but the direct cell division stimulus that brings about nodule primordia …
nodule symbiosis, but the direct cell division stimulus that brings about nodule primordia …
[HTML][HTML] Designer diatom episomes delivered by bacterial conjugation
Eukaryotic microalgae hold great promise for the bioproduction of fuels and higher value
chemicals. However, compared with model genetic organisms such as Escherichia coli and …
chemicals. However, compared with model genetic organisms such as Escherichia coli and …
[HTML][HTML] Genetic requirements for cell division in a genomically minimal cell
JF Pelletier, L Sun, KS Wise, N Assad-Garcia, BJ Karas… - Cell, 2021 - cell.com
Genomically minimal cells, such as JCVI-syn3.0, offer a platform to clarify genes underlying
core physiological processes. Although this minimal cell includes genes essential for …
core physiological processes. Although this minimal cell includes genes essential for …
An Expanded Plasmid-Based Genetic Toolbox Enables Cas9 Genome Editing and Stable Maintenance of Synthetic Pathways in Phaeodactylum tricornutum
…, Z Klassen, I Desgagné-Penix, BJ Karas… - ACS synthetic …, 2018 - ACS Publications
With the completion of the genome sequence, and development of an efficient conjugation-based
transformation system allowing the introduction of stable episomes, Phaeodactylum …
transformation system allowing the introduction of stable episomes, Phaeodactylum …
Carbonate-sensitive phytotransferrin controls high-affinity iron uptake in diatoms
In vast areas of the ocean, the scarcity of iron controls the growth and productivity of
phytoplankton 1 , 2 . Although most dissolved iron in the marine environment is complexed with …
phytoplankton 1 , 2 . Although most dissolved iron in the marine environment is complexed with …
[HTML][HTML] Efficient inter-species conjugative transfer of a CRISPR nuclease for targeted bacterial killing
…, JA Therrien, DT Ham, PC Bartlett, BJ Karas… - Nature …, 2019 - nature.com
The selective regulation of bacteria in complex microbial populations is key to controlling
pathogenic bacteria. CRISPR nucleases can be programmed to kill bacteria, but require an …
pathogenic bacteria. CRISPR nucleases can be programmed to kill bacteria, but require an …
Assembly of large, high G+ C bacterial DNA fragments in yeast
The ability to assemble large pieces of prokaryotic DNA by yeast recombination has great
application in synthetic biology, but cloning large pieces of high G+C prokaryotic DNA in yeast …
application in synthetic biology, but cloning large pieces of high G+C prokaryotic DNA in yeast …
Valproate tremors
BJ Karas, BJ Wilder, EJ Hammond, AW Bauman - Neurology, 1982 - AAN Enterprises
We made accelerometric recordings of the tremor induced by valproic acid. The tremor was
similar to essential tremor and appeared within a month of starting therapy. It was present at …
similar to essential tremor and appeared within a month of starting therapy. It was present at …
Technological challenges and milestones for writing genomes
Engineering biology with recombinant DNA, broadly called synthetic biology, has progressed
tremendously in the last decade, owing to continued industrialization of DNA synthesis, …
tremendously in the last decade, owing to continued industrialization of DNA synthesis, …