… screen, IN-HATw and IN-HATm were then cloned in a yeast expression vector (PBD-Gal4)
in-frame with GAL4 DNA binding domain (GDBD) at the N terminus and a HA tag at the C …

… of the proline-rich region favours Tau localization mainly in the soma and in the dendritic
compartment, whereas, both the dephosphorylation of PRD and the phosphorylation in the C-…

… (C) Chromatin immunoprecipitation (ChIP) analysis of viral DNA associated with γ-H2AX.
(Upper) Schematic representation of the positions of the primers used for real… Cristina Di Primio …

… was 40 cycles consisting of 95C for 30 s, 60C for 30 s, and 72C for 30 s. … C for 2 min and
95C for 10 min, followed by 40 cycles of denaturation at 95C for 15 s and extension at 60C …

… The PCR conditions were as follows: 42C for 2 min, 45 cycles of 95C for 10 s, annealing at
95C for 5 s, and elongation at 62C for 30 s, followed by a final elongation at 72C for 10 min. …

… (95C during 5 seconds, 60C during 15 seconds and 72C during 15 seconds or 95C for 15
seconds and 58C … 5 minutes at 65C for annealing primer to the template RNA, 2 minutes at 50…

… at 95 C for 15 s, annealing at 60 C for 25 s and extension at 72 C for 20 s. The primers
employed were as follows: Actin: fwd 5′-TCCATCCTGGCCTCACTGTCCAC-3′, rev 5′-…

… Briefly, the CST is the fusion of the Tau protein with the CFP at the C-terminal and the YFP
at the N-terminal. In normal conditions, the binding to MTs induces the folding of the CST into …

The central events of HIV-1 life cycle occur at the nuclear level where the viral genome is
integrated into the host cellular DNA in order to be expressed and replicated. The viral pre-…

… Cristina Di Primio Laboratorio di Terapia Genica e Molecolare, Istituto di Fisiologia Clinica
… 6% of amino acid, and there is an uncharacterised C-terminal tract present only in the yeast …