User profiles for C. Eggeling

Christian Eggeling

Professor of Super-Resolution Microscopy, University Jena Germany & University of Oxford
Verified email at rdm.ox.ac.uk
Cited by 30945

The mystery of membrane organization: composition, regulation and roles of lipid rafts

E Sezgin, I Levental, S Mayor, C Eggeling - Nature reviews Molecular …, 2017 - nature.com
c | Various probes can be used to study raft domains. Domain-selective probes partition
into one of the domains, whereas domain-sensitive probes partition to both domains and …

Super-resolution microscopy demystified

L Schermelleh, A Ferrand, T Huser, C Eggeling… - Nature cell …, 2019 - nature.com
… 4d) that correlate with cytochrome C release and may be required for the established role
of Bax in mitochondrial outer membrane permeabilization. Numerous other publications have …

Data registration and selective single-molecule analysis using multi-parameter fluorescence detection

C Eggeling, S Berger, L Brand, JR Fries… - Journal of …, 2001 - Elsevier
… P 1 C t ,g =∫P 1 C t ,g,t Pt 1 (t) d t , over all dwell times t. The probability, P n (C t ,g), to
register C t … convolution of P 1 (C t ,g) (eg P 2 (C t ,g)=P 1 (C t ,g)⊗P 1 (C t ,g), P 3 (C t ,g)=P 2 (…

Direct observation of the nanoscale dynamics of membrane lipids in a living cell

C Eggeling, C Ringemann, R Medda, G Schwarzmann… - Nature, 2009 - nature.com
… We performed most measurements at 27–37 C in HDMEM as outlined in detail in the
Supplementary Information. We excluded diffusion of non-integrated lipids (or dye tags) by control …

Photobleaching of fluorescent dyes under conditions used for single-molecule detection: Evidence of two-step photolysis

C Eggeling, J Widengren, R Rigler… - Analytical …, 1998 - ACS Publications
… k ads and k des , leading to a dye concentration c solv in solution and c ads on the surface,
and (2) irreversible photobleaching of the free dye with the rate constant due to illumination: …

Fluorescence nanoscopy by ground-state depletion and single-molecule return

…, R Medda, CA Wurm, B Hein, S Jakobs, C Eggeling… - Nature …, 2008 - nature.com
We introduce far-field fluorescence nanoscopy with ordinary fluorophores based on switching
the majority of them to a metastable dark state, such as the triplet, and calculating the …

A near-infrared fluorophore for live-cell super-resolution microscopy of cellular proteins

…, C Schultz, EA Lemke, P Heppenstall, C Eggeling… - Nature …, 2013 - nature.com
c, GSDIM/STORM images reconstructed from 10,000 raw images (c′ was taken ten minutes
after c). The enhancement in resolution permitted the detection of substructures. Scale bar, …

STED microscopy reveals crystal colour centres with nanometric resolution

E Rittweger, KY Han, SE Irvine, C Eggeling, SW Hell - Nature Photonics, 2009 - nature.com
… /√(C /I S ), with the constant C depending on the shape of the minimum 22 . Although the
resolution Δ x,y scales with the wavelength, adjusting ≫ I S squeezes the PSF continuously, …

Breaking the diffraction barrier in fluorescence microscopy at low light intensities by using reversibly photoswitchable proteins

M Hofmann, C Eggeling, S Jakobs… - Proceedings of the …, 2005 - National Acad Sciences
… (c) Effect of I y on the time t on needed for settling the … c shows the weighted subtraction
of a from b. d exhibits the … g compares line profiles from the images a and c, extracted at the …

Anatomy and dynamics of a supramolecular membrane protein cluster

…, B Harke, G Donnert, B Rammner, C Eggeling… - Science, 2007 - science.org
… Inset in (B) shows exemplary interaction potentials for various cluster sizes n c at n max =
140 (15). Attractive interactions occur at n c < n max . Insets in lower images of (A) (length of …