For several decades the pathobiology of diabetic retinopathy has been the object of conjecture
and hypotheses. Indeed, very little was known about the cellular events triggered by …

10.7554/eLife.01749.001 Many studies are uncovering functional roles for long noncoding
RNAs (lncRNAs), yet few have been tested for in vivo relevance through genetic ablation in …

… , and stored at –20C. The eyes destined for retinal protein extraction were kept at 4C by the
eye … centrifuged at 16,000g for 15 min at 4C. The supernatant was collected and stored at –80…

… at −20C. The whole mounts were dehydrated through graded alcohols, defatted in xylene
overnight at 4C, … Lorenzi M, Gerhardinger C: Early cellular and molecular changes induced by …

… factor C/EBPδ (CCAAT/enhancer-binding protein δ). The induction of C/EBPδ by proinflammatory
cytokines is an essential step in the acute-phase response. Thus, upregulation of C/…

Diabetes of even short duration accelerates the death of capillary cells and neurons in the
inner retina by a process consistent with apoptosis. We examined whether the process is …

Realizing the full utility of brain organoids to study human development requires understanding
whether organoids precisely replicate endogenous cellular and molecular events, …

… Suspended cells were centrifuged at 200g for 5 min at 4C, resuspended in PBS, and smeared
… Lorenzi M, Gerhardinger C: Early cellular and molecular changes induced by diabetes in …

… (C) Wilcoxon effect size differences between lincRNAs and mRNAs across several gene/promoter
properties. The genes analyzed are the same as in A. (D) Fisher effect size differences …

… g for 15 min at 4C, and the supernatant was collected and stored in aliquots at −80C. Protein
… Lorenzi M, Gerhardinger C: Early cellular and molecular changes induced by diabetes in …