… an isopeptide linkage between the ubiquitin C terminus and the ɛ-… forms a thioester with the
ubiquitin C terminus, and is then … In contrast, the homology to E6AP C terminus (HECT) and …

… Hawse & C. Wolberger, unpublished data) suggests that modest revisions to this scheme …
core at the N and C termini. Human SirT1, for example, has ∼240 amino acid N- and …

… repressor recognition helix is inserted into the major groove with its N-terminal portion
embedded more deeply than the C-terminal end, which tilts away from the DNA (Figure 1A). Other …

Sir2 is an NAD-dependent deacetylase that connects metabolism with longevity in yeast,
flies, and worms. Mammals have seven Sir2 homologs (SIRT1–7). We show that SIRT4 is a …

… All reactions were incubated at 30C except CobB reactions (37C) and Sir2AF reactions (55C…
Extracts were prepared from 100 ml cultures grown in yeast extract/peptone/dextrose at 30C …

… (C) The kcat values of bacterially produced Histagged recombinant Nampt and intra- and
extracellular Nampt-FLAG from NIH 3T3 cells and differentiated HIB-1B cells were calculated …

A common point of reference is needed to describe the three-dimensional arrangements of
bases and base-pairs in nucleic acid structures. The different standards used in computer …

… Figure 1C). It has been a matter of debate as to whether the C pocket is also used in the
show that free nicotinamide binds in the same conserved C pocket in which NAD+ is activated …

… (c) View as in a but rotated 90 around the horizontal axis looking down at the top of the
molecule in a. Representations of nucleosomes were generated using PyMOL (http://www.pymol.…

… by covalent bonds between the C terminus of one ubiquitin and … The ubiquitination cascade
begins when the C-terminal … an isopeptide bond with the C terminus of ubiquitin. This final …