Cell polarity is the ultimate reflection of complex mechanisms that establish and maintain
functionally specialized domains in the plasma membrane and cytoplasm. The spatial …

Actin polymerization often occurs at the plasma membrane to drive the protrusion of lamellipodia
and filopodia at the leading edge of migrating cells. A role for actin polymerization in …

… Carreno, JE Heuser & DG Drubin, manuscript in preparation). To better understand the
function of Hip1R and to gain insights into the function of the actin cytoskeleton in endocytosis, …

Tau protein from mammalian brain promotes microtubule polymerization in vitro and is
induced during nerve cell differentiation. However, the effects of tau or any other microtubule-…

We report that the actin assembly inhibitor latrunculin-A (LAT-A) causes complete disruption
of the yeast actin cytoskeleton within 2–5 min, suggesting that although yeast are nonmotile, …

Endocytosis depends on an extensive network of interacting proteins that execute a series
of distinct subprocesses. Previously, we used live-cell imaging of six budding-yeast proteins …

In budding yeast, many proteins involved in endocytic internalization, including adaptors and
actin cytoskeletal proteins, are localized to cortical patches of differing protein composition. …

The Aurora kinase Ipl1p plays a crucial role in regulating kinetochore-microtubule attachments
in budding yeast, but the underlying basis for this regulation is not known. To identify …

Publisher Summary This chapter provides protocols for the application of immunofluorescence
procedures to yeast. It should perhaps be stressed that immunofluorescence and other …

… We are grateful to members of the Barnes and Drubin laboratories, Fred Chang, and David
… This work was supported by grants awarded to G Barnes and DG Drubin by the National …