User profiles for D. W. Morgens

David Morgens

University of California, Berkeley
Verified email at berkeley.edu
Cited by 4388

CMTM6 maintains the expression of PD-L1 and regulates anti-tumour immunity

…, S Stolzenburg, O Gilan, S Bloor, T Noori, DW Morgens… - Nature, 2017 - nature.com
Cancer cells exploit the expression of the programmed death-1 (PD-1) ligand 1 (PD-L1) to
subvert T-cell-mediated immunosurveillance 1 , 2 . The success of therapies that disrupt PD-…

Lipid-droplet-accumulating microglia represent a dysfunctional and proinflammatory state in the aging brain

…, JV Pluvinage, V Mathur, O Hahn, DW Morgens… - Nature …, 2020 - nature.com
Microglia become progressively activated and seemingly dysfunctional with age, and
genetic studies have linked these cells to the pathogenesis of a growing number of …

Synergistic drug combinations for cancer identified in a CRISPR screen for pairwise genetic interactions

K Han, EE Jeng, GT Hess, DW Morgens, A Li… - Nature …, 2017 - nature.com
Identification of effective combination therapies is critical to address the emergence of drug-resistant
cancers, but direct screening of all possible drug combinations is infeasible. Here …

Systematic comparison of CRISPR/Cas9 and RNAi screens for essential genes

DW Morgens, RM Deans, A Li, MC Bassik - Nature biotechnology, 2016 - nature.com
We compared the ability of short hairpin RNA (shRNA) and CRISPR/Cas9 screens to identify
essential genes in the human chronic myelogenous leukemia cell line K562. We found that …

[HTML][HTML] Genome-scale measurement of off-target activity using Cas9 toxicity in high-throughput screens

DW Morgens, M Wainberg, EA Boyle, O Ursu… - Nature …, 2017 - nature.com
CRISPR-Cas9 screens are powerful tools for high-throughput interrogation of genome function,
but can be confounded by nuclease-induced toxicity at both on- and off-target sites, likely …

CD22 blockade restores homeostatic microglial phagocytosis in ageing brains

…, J Sun, T Iram, L Bonanno, L Li, DP Lee, DW Morgens… - Nature, 2019 - nature.com
Microglia maintain homeostasis in the central nervous system through phagocytic clearance
of protein aggregates and cellular debris. This function deteriorates during ageing and …

CRISPR–Cas9 screens in human cells and primary neurons identify modifiers of C9ORF72 dipeptide-repeat-protein toxicity

NJ Kramer, MS Haney, DW Morgens, A Jovičić… - Nature …, 2018 - nature.com
Hexanucleotide-repeat expansions in the C9ORF72 gene are the most common cause of
amyotrophic lateral sclerosis and frontotemporal dementia (c9ALS/FTD). The nucleotide-…

Parallel shRNA and CRISPR-Cas9 screens enable antiviral drug target identification

RM Deans, DW Morgens, A Ökesli, S Pillay… - Nature chemical …, 2016 - nature.com
Broad-spectrum antiviral drugs targeting host processes could potentially treat a wide range
of viruses while reducing the likelihood of emergent resistance. Despite great promise as …

Identification of phagocytosis regulators using magnetic genome-wide CRISPR screens

MS Haney, CJ Bohlen, DW Morgens, JA Ousey… - Nature …, 2018 - nature.com
Phagocytosis is required for a broad range of physiological functions, from pathogen defense
to tissue homeostasis, but the mechanisms required for phagocytosis of diverse substrates …

A CRISPR-based screen for Hedgehog signaling provides insights into ciliary function and ciliopathies

DK Breslow, S Hoogendoorn, AR Kopp, DW Morgens… - Nature …, 2018 - nature.com
Primary cilia organize Hedgehog signaling and shape embryonic development, and their
dysregulation is the unifying cause of ciliopathies. We conducted a functional genomic screen …