Reverse micelles are nanometer-sized (1–10 nm) water droplets dispersed in organic media
obtained by the action of surfactants. Surfactant molecules organize with the polar part to …

Reactive oxygen species (ROS) are produced continuously throughout the cell as products
of various redox reactions. Yet these products function as important signal messengers, …

Endoplasmic reticulum (ER) oxidation 1 (ERO1) transfers disulfides to protein disulfide
isomerase (PDI) and is essential for oxidative protein folding in simple eukaryotes such as yeast …

The copper content of recombinant CotA laccase from Bacillus subtilis produced by Escherichia
coli cells is shown to be strongly dependent on the presence of copper and oxygen in …

Site-directed mutagenesis has been used to replace Met502 in CotA laccase by the residues
leucine and phenylalanine. X-ray structural comparison of M502L and M502F mutants with …

Interfering with disulfide bond formation impedes protein folding and promotes endoplasmic
reticulum (ER) stress. Due to limitations in measurement techniques, the relationships of …

C-glycosides are natural products with important biological activities but are recalcitrant to
degradation. Glycoside 3-oxidases (G3Oxs) are recently identified bacterial flavo-oxidases …

The axial ligand of the catalytic mononuclear T1 copper site (Met 502 ) of the CotA laccase
was replaced by a leucine or phenylalanine residue to increase the redox potential of the …

Background The fate of hydrogen peroxide (H 2 O 2 ) in the endoplasmic reticulum (ER) has
been inferred indirectly from the activity of ER-localized thiol oxidases and peroxiredoxins, …

This work describes a methodology to monitor protein unfolding by using the well known
changes in tyrosine absorbance with the ionization of the side chain phenol group. It can be …