Regulation of virulence gene expression in Vibrio cholerae by quorum sensing: HapR functions at the aphA promoter

G Kovacikova, K Skorupski - Molecular microbiology, 2002 - Wiley Online Library
… 7, changing the T at position −85 to a G in the C6706 promoter fragment had no effect on …
Influence of the C6706 G-77T change on virulence gene expression As the G-77T change in …

A Vibrio cholerae LysR Homolog, AphB, Cooperates with AphA at the tcpPH Promoter To Activate Expression of the ToxR Virulence Cascade

G Kovacikova, K Skorupski - Journal of bacteriology, 1999 - Am Soc Microbiol
We describe here a new member of the LysR family of transcriptional regulators, AphB, which
is required for activation of the Vibrio cholerae ToxR virulence cascade. AphB activates the …

Overlapping binding sites for the virulence gene regulators AphA, AphB and cAMP‐CRP at the Vibrio cholerae tcpPH promoter

G Kovacikova, K Skorupski - Molecular microbiology, 2001 - Wiley Online Library
… The A at −65 that is critical for transcriptional activation by AphB lies within the dyad, and
the presence of a G in this position in the El Tor promoter disrupts the symmetry of the site. …

A selective review of glutamate pharmacological therapy in obsessive–compulsive and related disorders

MA Grados, EB Atkins, GI Kovacikova… - … research and behavior …, 2015 - Taylor & Francis
… Citation55 In a placebo-controlled double-blind study of OCD, Citation56 24 subjects
were assigned glycine 60 g/day or placebo. Glycine noncompliance, partly due a lack of …

Characterization of Vibrio cholerae O1 El Tor biotype variant clinical isolates from Bangladesh and Haiti, including a molecular genetic analysis of virulence genes

MS Son, CJ Megli, G Kovacikova, F Qadri… - Journal of clinical …, 2011 - Am Soc Microbiol
… A missense mutation of an A 266 G in the coding region, resulting in an amino acid change
from asparagine to serine (N 89 S), was found in Bgd3, Bgd4, Bgd5, Bgd6, MQ1795, MQ04, …

The LysR-Type Virulence Activator AphB Regulates the Expression of Genes in Vibrio cholerae in Response to Low pH and Anaerobiosis

G Kovacikova, W Lin, K Skorupski - Journal of bacteriology, 2010 - Am Soc Microbiol
AphB is a LysR-type activator that initiates the expression of the virulence cascade in Vibrio
cholerae by cooperating with the quorum-sensing-regulated activator AphA at the tcpPH …

Dual regulation of genes involved in acetoin biosynthesis and motility/biofilm formation by the virulence activator AphA and the acetate‐responsive LysR‐type …

G Kovacikova, W Lin, K Skorupski - Molecular microbiology, 2005 - Wiley Online Library
… Sequencing of the resulting products revealed that the transcriptional start of alsD is an A
27 bp upstream of its ATG and that of alsR is either an A or a G 33–34 bp upstream of its ATG (…

The Alternative Sigma Factor σE Plays an Important Role in Intestinal Survival and Virulence in Vibrio cholerae

G Kovacikova, K Skorupski - Infection and immunity, 2002 - Am Soc Microbiol
… Sequencing of the major resulting PCR product revealed a transcriptional start site (G) 93 nt
… of it revealed a second transcriptional start site (G or C) 309 to 310 nt upstream of the ATG …

The quorum sensing regulator HapR downregulates the expression of the virulence gene transcription factor AphA in Vibrio cholerae by antagonizing Lrp‐ and VpsR …

W Lin, G Kovacikova, K Skorupski - Molecular microbiology, 2007 - Wiley Online Library
… naturally occurring G-77T change … Kovacikova and Skorupski, 2002a) because in both of
these strains, HapR is unable to repress the aphA promoter. However, the Δlrp mutation in the G

Integration Host Factor Positively Regulates Virulence Gene Expression in Vibrio cholerae

E Stonehouse, G Kovacikova, RK Taylor… - Journal of …, 2008 - Am Soc Microbiol
… Unlike H-NS, IHF recognizes a specific asymmetric site on DNA, the 13-bp consensus
target sequence WATCAANNNNTTR, where W represents A or T and R represents A or G (10). …