Bacteria and archaea have evolved adaptive immune defenses, termed clustered regularly
interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) systems, that use …

Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-associated
(Cas) systems in bacteria and archaea use RNA-guided nuclease activity to provide …

Rapid advances in DNA synthesis techniques have made it possible to engineer viruses,
biochemical pathways and assemble bacterial genomes. Here, we report the synthesis of a …

We describe complete design of a synthetic eukaryotic genome, Sc2.0, a highly modified
Saccharomyces cerevisiae genome reduced in size by nearly 8%, with 1.1 megabases of the …

RNA-guided gene drives capable of spreading genomic alterations made in laboratory
organisms through wild populations could be used to address environmental and public health …

High-frequency oligonucleotide-directed recombination engineering (recombineering) has
enabled rapid modification of several prokaryotic genomes to date. Here, we present a …

Candida albicans is the leading cause of fungal infections; yet, complex genetic interaction
analysis remains cumbersome in this diploid pathogen. Here, we developed a CRISPR–Cas9…

Precision medicine seeks to treat disease with molecular specificity. Advances in genome
sequence analysis, gene delivery, and genome surgery have allowed clinician-scientists to …

Construction and characterization of large genetic variant libraries is essential for understanding
genome function, but remains challenging. Here, we introduce a Cas9-based approach …

Over the past year, CRISPR-Cas genome editing has been used to correct pathogenic
mutations in vivo and in transplantable stem cells. Although off-target mutagenesis remains a …