Resveratrol has gained tremendous interest owing to multiple reported health-beneficial
effects. However, the underlying key mechanism of action of this natural product remained …

… Furthermore, human Grx1 reacts twenty times faster with GSH than with L-cysteinylglycine
or L-cysteine, revealing that the preference for GSH also depends on its γ-glutamyl moiety 19 . …

Class I glutaredoxins are enzymatically active, glutathione-dependent oxidoreductases,
whilst class II glutaredoxins are typically enzymatically inactive, Fe-S cluster-binding proteins. …

The human endogenous retrovirus family HERV-K(HML-2) Rec protein is an RNA transport
factor that enhances nuclear export of intron-containing retroviral transcripts. Using the yeast …

… redox interactome of LtErv, we generated L. tarentolae cell lines that chromosomally encode
… for L. donovani (35), we identified a suitable medium and established a SILAC protocol for L…

… Cultivation of L. tarentolae in standard BHI medium resulted … For inducible knock-downs, we
evaluated the growth of L. … the disruption and tagging of genes in L. tarentolae as well as the …

… In order to test the relevance of Erv in Leishmania, we tried to disrupt the encoding gene in
the human pathogen L. infantum (locus LinJ.15.1020) by homologous recombination (Fig. 1A)…

The enzymes glyoxalase 1 and 2 (Glo1 and Glo2) are found in most eukaryotes and catalyze
the glutathione-dependent conversion of 2-oxoaldehydes to 2-hydroxycarboxylic acids. …

This chapter reviews the classification of glutaredoxins (Grx) and introduces the diversity of
Grx substrates and enzyme assays followed by a discussion of the enzymatic mechanism of …

… Cell pellets from 1 L culture were rapidly resuspended in 15 mL ice-cold disruption buffer
(… -nitrilotriacetic acid agarose (Qiagen) per 0.5 L of culture. The columns were subsequently …