… (n = 3, ± SD for values above 8 mM; n = 2, ± SD for values below 8 mM) (see also Figure S3).
… shown in brown (n = 3, ± SD for values above 8 mM; n = 2, ± SD for values below 8 mM). …

Can a heterotrophic organism be evolved to synthesize biomass from CO 2 directly? So far,
non-native carbon fixation in which biomass precursors are synthesized solely from CO 2 …

… Where each plasmid contains the same N coding sequences in a pre-defined order but which
are driven by a varying combination of the six RBS sequences ( Supplementary Figures S6…

… coli platform, two rounds of directed evolution led to the identification of a novel point
mutation, localized at the interface between the N-terminal and C-terminal domains of the large …

CO 2 is converted into biomass almost solely by the enzyme rubisco. The poor carboxylation
properties of plant rubiscos have led to efforts that made it the most kinetically characterized …

Translational coupling is the interdependence of translation efficiency of neighboring genes
encoded within an operon. The degree of coupling may be quantified by measuring how the …

Many photosynthetic organisms employ a CO 2 concentrating mechanism (CCM) to
increase the rate of CO 2 fixation via the Calvin cycle. CCMs catalyze ≈50% of global …

10.7554/eLife.20667.001 A set of chemical reactions that require a metabolite to synthesize
more of that metabolite is an autocatalytic cycle. Here, we show that most of the reactions in …

Pyruvate formate-lyase (PFL) is a ubiquitous enzyme that supports increased ATP yield during
sugar fermentation. While the PFL reaction is known to be reversible in vitro, the ability of …

… as either novel (n = 44) or related to a previously sequenced BGC (n = 16; Supplementary
Fig. … in the 60 clones we recovered, over 95% (n = 237) were found on the metagenomic DNA …