Fluorescence in situ hybridization (FISH), the assay of choice for localization of specific
nucleic acids sequences in native context, is a 20-year-old technology that has developed …

The spatial regulation of protein translation is an efficient way to create functional and structural
asymmetries in cells. Recent research has furthered our understanding of how individual …

… Subcellular mRNA localization was first reported in 1986 by Lawrence and Singer, who
observed this phenomenon in chicken fibroblasts using in situ hybridization 5 , and was …

… To determine whether mRNAs exhibit random or nonrandom distribution within cells, we
have used an improved in situ hybridization methodology (Lawrence and Singer, 1985) …

… transport field, as well as addressing immediate quesEphrussi, 1996; Steward and Singer, …
Long, RM, Elliott, DJ, Stutz, F., Rosbash, M., and Singer, RH a myosin and restricts yeast …

… The FISH protocol was modified from KL Taneja and RH Singer [J. Cell. Biochem. … AM
Femino and RH Singer, Department of Anatomy and Structural Biology and Cell Biology, Albert …

Specific labeling of biomolecules with bright fluorophores is the keystone of fluorescence
microscopy. Genetically encoded self-labeling tag proteins can be coupled to synthetic dyes …

… Sundell and Singer have previously reported that actin mRNA in the lamellae appears in
the light … Sundell, C., and RH Singer. 1991. Requirement of microfilaments in sorting of actin …

Proper execution of transcriptional programs is a key requirement of gene expression
regulation, demanding accurate control of timing and amplitude. How precisely the transcription …

It has not been possible to view the transcriptional activity of a single gene within a living
eukaryotic cell. It is therefore unclear how long and how frequently a gene is actively transcribed…