Typical genotyping workflows map reads to a reference genome before identifying genetic
variants. Generating such alignments introduces reference biases and comes with substantial …

… x and seeded on T-25 or T-75 rat-tail collagen-coated tissue flasks (Greiner Bio-One,
Kremsmünster, Austria), according to the pellet size, respectively, and incubated at 37C, 5% CO 2 . …

… T nucleotide, considers the number of T to C conversions and the number of non T to C
conversions in the aligned reads. For each T nucleotide in the RNA sequence, the number of T to …

The primary problem with the explosion of biomedical datasets is not the data, not computational
resources, and not the required storage space, but the general lack of trained and …

We whole-genome sequenced 55 SARS-CoV-2 isolates from Germany to investigate SARS-CoV-2
outbreaks in 2020 in the Heinsberg district and Düsseldorf. While the genetic …

In optogenetics, light-activated proteins are used to monitor and modulate cellular behaviour
with light. Combining genetic targeting of distinct cellular populations with defined patterns …

What does it take to convert a heap of sequencing data into a publishable result? First,
common tools are employed to reduce primary data (sequencing reads) to a form suitable for …

Background Tracing of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
transmission chains is still a major challenge for public health authorities, when incidental …

… To study which system parameters determine the characteristic times of quenching activation,
we determine the half-times, T 1/2 , defined as the time the quenching variable N requires …

RNA-based regulation has become a major research topic in molecular biology. The analysis
of epigenetic and expression data is therefore incomplete if RNA-based regulation is not …