… Fundamentally, a particle can be stably trapped if the depth of the optically induced
potential is significantly greater than k B T, which at room temperature (290 K) is convenient to …

Recent developments in reagent design can address problems in single cells that were not
previously approachable. We have attempted to foresee what will become possible, and the …

… A fast mobile component showed that ∼75% of the molecules were diffusing freely and the
immobile component showed that ∼25% of the molecules were transiently immobile with a t …

Although messenger RNA (mRNA) translation is a fundamental biological process, it has
never been imaged in real time in vivo with single-molecule precision. To achieve this, we …

… (t–v) Analysis of blinking behavior of PA-JF 549 during PALM of ensconsin–HaloTag shown
in r. (t) Histogram of the duration of individual blinking events (magenta), overlaid with the fit …

Specific labeling of biomolecules with bright fluorophores is the keystone of fluorescence
microscopy. Genetically encoded self-labeling tag proteins can be coupled to synthetic dyes …

Enhancer-binding pluripotency regulators (Sox2 and Oct4) play a seminal role in embryonic
stem (ES) cell-specific gene regulation. Here, we combine in vivo and in vitro single-…

Live-cell single mRNA imaging is a powerful tool but has been restricted in higher eukaryotes
to artificial cell lines and reporter genes. We describe an approach that enables live-cell …

… We plotted the average Pol II cluster lifetime (at time t) against the number of nascent mRNA
subsequently transcribed (at time t+Dt). For the data comprising the b-actin gene response …

Direct visualization of genomic loci in the 3D nucleus is important for understanding the spatial
organization of the genome and its association with gene expression. Various DNA FISH …