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     Save 15% and Get Your Epitranscriptomic Profiling Done in the Right Way!


Only 5ug total RNA is required! Cover mRNA & LncRNA, or circRNA modifications.

Current transcriptome-wide RNA modification profiling methods deal mostly with mapping the modified sites but are unable to quantify the percentage of modified RNA for that transcript. The lack of such quantitative information has been a major concern for scientists [1,2].

Arraystar Epitranscriptomic Arrays combine the two-color channel microarray technology with RNA modification immunoprecipitation have the power to determine: which gene transcripts are modified, the modification quantity and the percentage of modification for each transcript (Table 1). mRNA&lncRNA Epitranscriptomic Microarray and circRNA Epitranscriptomic Microarray quantify your choice of m6A/m5C/m1A/ac4C/m7G/Ψ epitranscriptomic modification in mRNAs & lncRNAs or circRNAs.

“The modification quantity for each transcript”
  Raw intensity   m6A Quantity   Annotations
Gene
Symbol
IP Input IP(Corrected) Seqname
GJD4 25.43 22.04 13.85 NM_153368
NOP9 83.72 269.53 76.98 NM_174913
TM7SF3 73.85 948.20 46.71 NM_016551
RNF167 506.53 1150.78 643.45 NM_015528
TMR10 25.24 22.23 14.41 NM_017762
“The percentage of modification for each transcript”
  Raw intensity   m6A   Annotations
Gene
Symbol
IP Input %Modified Seqname
GJD4 25.43 22.04 45.1% NM_153368
NOP9 83.72 269.53 15.3% NM_174913
TM7SF3 73.85 948.20 3.2% NM_016551
RNF167 506.53 1150.78 15.8% NM_015528
MTMR10 25.24 22.23 44.5% NM_017762

Table 1. Epitranscriptomic Array detects the modified RNA in Cy5 and unmodified RNA in Cy3 channels on the same array, such that the modified percentages for each transcript can be measured. “The modification quantity” is calculated based on the normalized IP intensities, and “The percentage of modification” is calculated as IP/Total RNA.


Customer Publications in Epitranscriptomics

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1. Offer valid 02/01/2024 - 5/31/2024.
2. May not be combined with any other promotions or discounts.
3. Minimum order: 6 samples. Sample requirements: 5 ug total RNA in good quality.
4. Offer only valid for direct purchase from Arraystar Inc.




Selected Publications

• Circular RNA Array
   [PMID: 37004067] Zhong GL, et al.
   (2023) Molecular Cancer

• m6A Single Nucleotide Array
   [PMID: 36631441] Yan W, et al.
   (2023) International Journal of Oral
   Science

• tRNA PCR Array
   [PMID: 37283053] Wang C, et al.
   (2023) Nucleic Acids Research

• LC-MS Based tRNA Modification
   [PMID: 37563288] Li G, et al.
   (2023) Nature Microbiology


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