Biochemistry

Design: An assay based on single-polypeptide-chain heterodimeric A_2AR/D₂R and non-oligomerized fusions for in vivo analysis of their allosteric receptor-receptor interactions
Toshio Kamiya, Takashi Masuko, Dasiel Oscar Borroto-Escuela, Haruo Okado, Hiroyasu Nakata

bioRxiv 065250; doi: https://doi.org/10.1101/065250

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Beyond the myosin mesa: a potential unifying hypothesis on the underlying molecular basis of hyper-contractility caused by a majority of hypertrophic cardiomyopathy mutations
Suman Nag, Darshan V. Trivedi, Saswata S. Sarkar, Shirley Sutton, Kathleen M. Ruppel, James A. Spudich

bioRxiv 065508; doi: https://doi.org/10.1101/065508

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Acidic pH is a Metabolic Switch for 2-Hydroxyglutarate Generation and Signaling
Sergiy M. Nadtochiy, Xenia Schafer, Dragony Fu, Keith Nehrke, Joshua Munger, Paul S. Brookes

bioRxiv 051599; doi: https://doi.org/10.1101/051599

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The Origin of Coupled Chloride and Proton Transport in a Cl⁻/H⁺ Antiporter
Sangyun Lee, Heather B. Mayes, Jessica M. J. Swanson, Gregory A. Voth

bioRxiv 064527; doi: https://doi.org/10.1101/064527

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Zika virus may not be alone: proteomics associates a bovine-like viral diarrhea virus to microcephaly
Fabio CS Nogueira, Erika Velasquez, Adriana SO Melo, Gilberto B Domont, Akira Sawa

bioRxiv 062596; doi: https://doi.org/10.1101/062596 New Results

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The metabolic profile of lag and exponential phases of Saccharomyces cerevisiae growth changes continuously
Rafael N. Bento, Miguel A. Aradhya, Valdir A. R. Semedo, Carlos E. S. Bernardes, Manuel E. M. Piedade, Fernando Antunes

bioRxiv 063909; doi: https://doi.org/10.1101/063909

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Structural characterization of encapsulated ferritin provides insight into iron storage in bacterial nanocompartments
Didi He, Sam Hughes, Sally Vanden-Hehir, Atanas Georgiev, Kirsten Altenbach, Emma Tarrant, C. Logan Mackay, Kevin J. Waldron, David J. Clarke, Jon Marles-Wright

bioRxiv 063495; doi: https://doi.org/10.1101/063495

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Antiparallel protocadherin homodimers use distinct affinity- and specificity-mediating regions in cadherin repeats 1-4
John M. Nicoludis, Bennett E. Vogt, Anna G. Green, Charlotta P. I. Schärfe, Debora S. Marks, Rachelle Gaudet

bioRxiv 063289; doi: https://doi.org/10.1101/063289

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Ubiquitin-mediated proteolysis in Xenopus extract
Gary S. McDowell, Anna Philpott

bioRxiv 062331; doi: https://doi.org/10.1101/062331 Confirmatory Results

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High-throughput biochemical profiling reveals Cas9 off-target binding and unbinding heterogeneity
Evan A. Boyle, Johan O. L. Andreasson, Lauren M. Chircus, Samuel H. Sternberg, Michelle J. Wu, Chantal K. Guegler, Jennifer A. Doudna, William J. Greenleaf

bioRxiv 059782; doi: https://doi.org/10.1101/059782

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Biochemistry

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