Molecular Biology

Generation and validation of homozygous fluorescent knock-in cells using CRISPR/Cas9 genome editing
Birgit Koch, Bianca Nijmeijer, Moritz Kueblbeck, Yin Cai, Nike Walther, Jan Ellenberg

bioRxiv 188847; doi: https://doi.org/10.1101/188847

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Deficiency of the E3 Ubiquitin Ligase RBCK1 Causes Diffuse Brain Polyglucosan Accumulation and Neurodegeneration
Mitchell A. Sullivan, Felix Nitschke, Erin E. Chown, Laura F. DiGiovanni, Mackenzie Chown, Ami M. Perri, Sharmistha Mitra, Xiaochu Zhao, Cameron A. Ackerley, Lori Israelian, Saija Ahonen, Peixiang Wang, Berge A. Minassian

bioRxiv 277392; doi: https://doi.org/10.1101/277392

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Long-read sequencing of nascent RNA reveals coupling among RNA processing events
Lydia Herzel, Korinna Straube, Karla M. Neugebauer

bioRxiv 230334; doi: https://doi.org/10.1101/230334

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HIV-1 Nefs are cargo-sensitive AP-1 trimerization switches in tetherin and MHC-I downregulation
Kyle L. Morris, Cosmo Z. Buffalo, Christina M. Stürzel, Elena Heusinger, Frank Kirchhoff, Xuefeng Ren, James H. Hurley

bioRxiv 276733; doi: https://doi.org/10.1101/276733

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Needle in a haystack? A comparison of eDNA metabarcoding and targeted qPCR for detection of the great crested newt (Triturus cristatus)
Lynsey R. Harper, Lori Lawson Handley, Christoph Hahn, Neil Boonham, Helen C. Rees, Kevin C. Gough, Erin Lewis, Ian P. Adams, Peter Brotherton, Susanna Phillips, Bernd Hänfling

bioRxiv 215897; doi: https://doi.org/10.1101/215897

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A method for simultaneous targeted mutagenesis of all nuclear rDNA repeats in Saccharomyces cerevisiae using CRISPR-Cas9
Lilly Chiou, Daniele Armaleo

bioRxiv 276220; doi: https://doi.org/10.1101/276220

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The genetic intractability of Symbiodinium microadriaticum to standard algal transformation methods
Jit Ern Chen, Adrian C. Barbrook, Guoxin Cui, Christopher J. Howe, Manuel Aranda

bioRxiv 140616; doi: https://doi.org/10.1101/140616

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A stable mode of bookmarking by TBP recruits RNA Polymerase II to mitotic chromosomes
Sheila S. Teves, Luye An, Aarohi Bhargava-Shah, Liangqi Xie, Xavier Darzacq, Robert Tjian

bioRxiv 257451; doi: https://doi.org/10.1101/257451

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mRNA structure dynamics identifies the embryonic RNA regulome
Jean-Denis Beaudoin, Eva Maria Novoa, Charles E Vejnar, Valeria Yartseva, Carter Takacs, Manolis Kellis, Antonio J Giraldez

bioRxiv 274290; doi: https://doi.org/10.1101/274290

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High-precision CRISPR-Cas9 base editors with minimized bystander and off-target mutations
Jason M. Gehrke, Oliver Cervantes, M. Kendell Clement, Luca Pinello, J. Keith Joung

bioRxiv 273938; doi: https://doi.org/10.1101/273938

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Molecular Biology

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