Synthetic Biology

From empirical to data-driven host selection: a broad-host-range expression platform to facilitate chassis screening
Viviënne Mol, Kristoffer Bach Falkenberg, Ácil De Almeida Will, Ivan Pogrebnyakov, Charlotte Beck, Anna Lyhne Skøttrup, Alex Toftgaard Nielsen, Sheila Ingemann Jensen

bioRxiv 2022.08.09.503317; doi: https://doi.org/10.1101/2022.08.09.503317 New Results

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Implementation of the β-hydroxyaspartate cycle increases growth performance of Pseudomonas putida on the PET monomer ethylene glycol
Lennart Schada von Borzyskowski, Helena Schulz-Mirbach, Mauricio Troncoso Castellanos, Francesca Severi, Paul A. Gomez Coronado, Timo Glatter, Arren Bar-Even, Steffen N. Lindner, Tobias J. Erb

bioRxiv 2022.08.08.503134; doi: https://doi.org/10.1101/2022.08.08.503134 New Results

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Inteins in the Loop: A Framework for Engineering Advanced Biomolecular Controllers for Robust Perfect Adaptation
Stanislav Anastassov, Maurice Filo, Ching-Hsiang Chang, Mustafa Khammash

bioRxiv 2022.08.05.502923; doi: https://doi.org/10.1101/2022.08.05.502923 New Results

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Novel genetic modules encoding high-level antibiotic-free protein expression in probiotic lactobacilli
Sourik Dey, Marc Blanch Asensio, Sanjana Balaji Kuttae, Shrikrishnan Sankaran

bioRxiv 2022.08.04.502766; doi: https://doi.org/10.1101/2022.08.04.502766 New Results

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Precision engineering of biological function with large-scale measurements and machine learning
Drew S. Tack, Peter D. Tonner, Abe Pressman, Nathanael D. Olson, Sasha F. Levy, Eugenia F. Romantseva, Nina Alperovich, Olga Vasilyeva, David Ross

bioRxiv 2022.08.04.502789; doi: https://doi.org/10.1101/2022.08.04.502789 New Results

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From peptides to proteins: coiled-coil tetramers to single-chain 4-helix bundles
Elise A. Naudin, Katherine I. Albanese, Abigail J. Smith, Bram Mylemans, Emily G. Baker, Orion D. Weiner, David M. Andrews, Natalie Tigue, Nigel J. Savery, Derek N. Woolfson

bioRxiv 2022.08.04.502660; doi: https://doi.org/10.1101/2022.08.04.502660 New Results

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Quantitative assaying of SpCas9-NG with fluorescent reporters
Alexandre Baccouche, Kevin Montagne, Nozomu Yachie, Teruo Fujii, Anthony Genot

bioRxiv 2022.08.04.502727; doi: https://doi.org/10.1101/2022.08.04.502727 New Results

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Dissecting the stability determinants of a challenging de novo protein fold using massively parallel design and experimentation
Tae-Eun Kim, Kotaro Tsuboyama, Scott Houliston, Cydney M. Martell, Claire M. Phoumyvong, Alexander Lemak, Hugh K. Haddox, Cheryl H. Arrowsmith, Gabriel J. Rocklin

bioRxiv 2021.12.17.472837; doi: https://doi.org/10.1101/2021.12.17.472837 New Results

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Rapid and accurate assembly of large DNA assisted by in vitro packaging of bacteriophage
Shingo Nozaki

bioRxiv 2022.08.01.502418; doi: https://doi.org/10.1101/2022.08.01.502418 New Results

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Pattern formation and bistability in a synthetic intercellular genetic toggle
Bárbara de Freitas Magalhães, Gaoyang Fan, Eduardo Sontag, Krešimir Josić, Matthew R. Bennett

bioRxiv 2022.08.02.502488; doi: https://doi.org/10.1101/2022.08.02.502488 New Results

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Synthetic Biology

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