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Synthetic Biology

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  • Deep learning of the regulatory grammar of yeast 5’ untranslated regions from 500,000 random sequences
    Josh Cuperus, Benjamin Groves, Anna Kuchina, Alexander B. Rosenberg, Nebojsa Jojic, Stanley Fields, Georg Seelig
    bioRxiv 137547; doi: https://doi.org/10.1101/137547
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  • Mobius Assembly: a versatile framework for Golden Gate assembly
    Andreas I. Andreou, Naomi Nakayama
    bioRxiv 140095; doi: https://doi.org/10.1101/140095
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  • Dynamic allocation of orthogonal ribosomes facilitates uncoupling of co-expressed genes
    Alexander P.S. Darlington, Juhyun Kim, José I. Jiménez, Declan G. Bates
    bioRxiv 138362; doi: https://doi.org/10.1101/138362
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  • Rational Design of RNA Structures that Predictably Tune Eukaryotic Gene Expression
    Tim Weenink, Robert M. McKiernan, Tom Ellis
    bioRxiv 137877; doi: https://doi.org/10.1101/137877
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  • Expression of Pokeweed Antiviral Protein Isoform S1 (PAP-S1) and of Ricin-A-Chain/PAP-S1 novel fusion protein (RTA/PAP-S1) in Escherichia coli and their comparative inhibition of protein synthesis in vitro
    Yasser Hassan, Sherry Ogg
    bioRxiv 137919; doi: https://doi.org/10.1101/137919
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  • Crosstalk between diverse synthetic protein degradation tags in Escherichia coli
    Nicholas C. Butzin, William H. Mather
    bioRxiv 136812; doi: https://doi.org/10.1101/136812
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  • Control of Lipid Domain Organization by a Biomimetic Contractile Actomyosin Cortex
    Sven K. Vogel, Ferdinand Greiss, Alena Khmelinskaia, Petra Schwille
    bioRxiv 096248; doi: https://doi.org/10.1101/096248
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  • Recombineering in Vibrio natriegens
    Henry H. Lee, Nili Ostrov, Michaela A. Gold, George M. Church
    bioRxiv 130088; doi: https://doi.org/10.1101/130088
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  • Rational Design of Evolutionarily Stable Microbial Kill Switches
    Finn Stirling, Lisa Bitzan, John WK Oliver, Jeffrey Way, Pamela A. Silver
    bioRxiv 129445; doi: https://doi.org/10.1101/129445
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  • The WPRE improves genetic engineering with site-specific nucleases
    Jessica M. Ong, Christopher R. Brown, Matthew C. Mendel, Gregory J. Cost
    bioRxiv 126904; doi: https://doi.org/10.1101/126904
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