Synthetic Biology

Development of a Freeze-Dried CRISPR-Cas12 Sensor for Detecting Wolbachia in the Secondary Science Classroom
Grant A. Rybnicky, Radeen A. Dixon, Robert M. Kuhn, Ashty S. Karim, Michael C. Jewett

bioRxiv 2021.10.06.463384; doi: https://doi.org/10.1101/2021.10.06.463384

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Exploiting noise, non-linearity, and feedback for differential control of multiple synthetic cells with a single optogenetic input
Michael P May, Brian Munsky

bioRxiv 2021.05.11.443599; doi: https://doi.org/10.1101/2021.05.11.443599

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Uncovering the distinct properties of a bacterial type I-E CRISPR activation system
Maria Claudia Villegas Kcam, Annette J. Tsong, James Chappell

bioRxiv 2021.10.06.463193; doi: https://doi.org/10.1101/2021.10.06.463193

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A cell-free assay for rapid screening of inhibitors of hACE2-receptor - SARS-CoV-2-Spike binding
Nanami Kikuchi, Or Willinger, Naor Granik, Noa Navon, Shanny Ackerman, Ella Samuel, Tomer Antman, Noa Katz, Sarah Goldberg, Roee Amit

bioRxiv 2021.10.06.462907; doi: https://doi.org/10.1101/2021.10.06.462907

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Heterologous Cas9 and non-homologous end joining as a Potentially Organism-Agnostic Knockout (POAK) system in bacteria
Isaac N. Plant

bioRxiv 601971; doi: https://doi.org/10.1101/601971

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Highly Reversible Tunable Thermal-repressible Split-T7 RNA polymerases (Thermal-T7RNAPs) for dynamic gene regulation
Wai Kit David Chee, Jing Wui Yeoh, Viet Linh Dao, Chueh Loo Poh

bioRxiv 2021.10.04.462087; doi: https://doi.org/10.1101/2021.10.04.462087

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Independent control of mean and noise by convolution of gene expression distributions
Karl P. Gerhardt, Satyajit D. Rao, Evan J. Olson, Oleg A. Igoshin, Jeffrey J. Tabor

bioRxiv 2021.01.21.427519; doi: https://doi.org/10.1101/2021.01.21.427519

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Genome-wide functional screens enable the prediction of high activity CRISPR-Cas9 and -Cas12a guides in Yarrowia lipolytica
Dipankar Baisya, Adithya Ramesh, Cory Schwartz, Stefano Lonardi, Ian Wheeldon

bioRxiv 2021.09.29.461753; doi: https://doi.org/10.1101/2021.09.29.461753

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De novo biosynthesis of para-nitro-L-phenylalanine in Escherichia coli
Neil D. Butler, Sabyasachi Sen, Minwei Lin, Aditya M. Kunjapur

bioRxiv 2021.09.29.462267; doi: https://doi.org/10.1101/2021.09.29.462267

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Transcriptional interference in toehold switch-based RNA circuits
Elisabeth Falgenhauer, Andrea Mückl, Matthaeus Schwarz-Schilling, Friedrich C. Simmel

bioRxiv 2021.09.29.462367; doi: https://doi.org/10.1101/2021.09.29.462367

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Synthetic Biology

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