Synthetic Biology

In vitro prototyping and rapid optimization of biosynthetic enzymes for cellular design
Ashty S. Karim, Quentin M. Dudley, Alex Juminaga, Yongbo Yuan, Samantha A. Crowe, Jacob T. Heggestad, Tanus Abdalla, William Grubbe, Blake Rasor, Dave Coar, Maria Torculas, Michael Krein, FungMin (Eric) Liew, Amy Quattlebaum, Rasmus O. Jensen, Jeff Stuart, Sean D. Simpson, Michael Köpke, Michael C. Jewett

bioRxiv 685768; doi: https://doi.org/10.1101/685768

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Computational design of anti-CRISPR proteins with improved inhibition potency and expanded specificity
Jan Mathony, Zander Harteveld, Carolin Schmelas, Julius Upmeier zu Belzen, Sabine Aschenbrenner, Mareike D. Hoffmann, Christina Stengl, Andreas Scheck, Stéphane Rosset, Dirk Grimm, Roland Eils, Bruno E. Correia, Dominik Niopek

bioRxiv 685032; doi: https://doi.org/10.1101/685032

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Chemogenetic Control of Nanobodies
Helen Farrants, Miroslaw Tarnawski, Thorsten G. Müller, Shotaro Otsuka, Julien Hiblot, Birgit Koch, Moritz Kueblbeck, Hans-Georg Kräusslich, Jan Ellenberg, Kai Johnsson

bioRxiv 683557; doi: https://doi.org/10.1101/683557

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Measuring glycolytic flux in single yeast cells with an orthogonal synthetic biosensor
Francisca Monteiro, Georg Hubmann, Justin Norder, Johan Hekelaar, Joana Saldida, Athanasios Litsios, Hein J. Wijma, Alexander Schmidt, Matthias Heinemann

bioRxiv 682302; doi: https://doi.org/10.1101/682302

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On-demand, cell-free biomanufacturing of conjugate vaccines at the point-of-care
Jessica C. Stark, Thapakorn Jaroentomeechai, Tyler D. Moeller, Rachel S. Dubner, Karen J. Hsu, Taylor C. Stevenson, Matthew P. DeLisa, Michael C. Jewett

bioRxiv 681841; doi: https://doi.org/10.1101/681841

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Cell-in-the-loop pattern formation with optogenetically emulated cell-to-cell signaling
Melinda Liu Perkins, Dirk Benzinger, Murat Arcak, Mustafa Khammash

bioRxiv 679597; doi: https://doi.org/10.1101/679597

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Recombinant Expression of Aldehyde Dehydrogenase 2 (ALDH2) in Escherichia coli Nissle 1917 for Oral Delivery in ALDH2-Deficient Individuals
Tim Ho, Catherine Chang, Justin Wu, Iris Huang, Leona Tsai, Justin Lin, Emily Tai, Caroline Chou, Justin Yang, Yvonne Wei, Catherine Yeh, William Chen, Dylan Lu, Charlotte Chou, Longan Su, Nicole Chang, Colin Huang, Chloe Wang, Paul Abrena, Christy Cheung, Cassandra Yeh, Shantih Whiteford, Phoebe Chen, Austin Huang, Aire Wu, Benjamin Wei, Eugene Kao, Nicholas Lin, Anna Chang, Jake Yang, Yasmin Lin, Sean Tsao, Nicholas Ward, Teresa Chiang, Jude Clapper

bioRxiv 674606; doi: https://doi.org/10.1101/674606

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Evaluating Protein Transfer Learning with TAPE
Roshan Rao, Nicholas Bhattacharya, Neil Thomas, Yan Duan, Xi Chen, John Canny, Pieter Abbeel, Yun S. Song

bioRxiv 676825; doi: https://doi.org/10.1101/676825

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A yeast optogenetic toolkit (yOTK) for gene expression control in Saccharomyces cerevisiae
Jidapas (My) An-adirekkun, Cameron J. Stewart, Stephanie H. Geller, Michael T. Patel, Justin Melendez, Benjamin L. Oakes, Marcus B. Noyes, Megan N. McClean

bioRxiv 663393; doi: https://doi.org/10.1101/663393

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Ab-initio discovery of tumoricidal oligonucleotides in a DNA sequencing machine
Noam Mamet, Itai Rusinek, Gil Harari, Zvi Shapira, Yaniv Amir, Erez Lavi, Adva Zamir, Noam Borovsky, Noah Joseph, Maria Motin, Dekel Saban, Ido Bachelet

bioRxiv 630830; doi: https://doi.org/10.1101/630830

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Synthetic Biology

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