Synthetic Biology

Directed evolution of an orthogonal transcription engine for programmable gene expression in eukaryotes
Shaunak Kar, Elizabeth C. Gardner, Kamyab Javanmardi, Daniel R. Boutz, Raghav Shroff, Andrew P. Horton, Thomas H. Segall-Shapiro, Andrew D. Ellington, Jimmy Gollihar

bioRxiv 2024.09.25.614978; doi: https://doi.org/10.1101/2024.09.25.614978 New Results

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Signal sequences target enzymes and structural proteins to bacterial microcompartments and are critical for microcompartment formation
Elizabeth R. Johnson, Nolan W. Kennedy, Carolyn. E. Mills, Shiqi Liang, Swetha Chandrasekar, Taylor M. Nichols, Grant A. Rybnicky, Danielle Tullman-Ercek

bioRxiv 2024.09.25.615066; doi: https://doi.org/10.1101/2024.09.25.615066 New Results

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De novo design of parallel and antiparallel A₃B₃ heterohexameric α-helical barrels
Joel J. Chubb, Katherine I. Albanese, Alison Rodger, Derek N. Woolfson

bioRxiv 2024.09.25.614870; doi: https://doi.org/10.1101/2024.09.25.614870 New Results

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Fundamental trade-offs in the robustness of biological systems with feedback regulation
Nguyen Hoai Nam Tran, An Nguyen, Tasfia Wasima Rahman, Ania-Ariadna Baetica

bioRxiv 2024.09.25.614654; doi: https://doi.org/10.1101/2024.09.25.614654 New Results

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Split RNA switch: Programmable and precise control of gene expression by ensemble of pre- and post-translational regulation
Itsuki Abe, Hirohisa Ohno, Megumi Mochizuki, Karin Hayashi, Hirohide Saito

bioRxiv 2024.09.25.614879; doi: https://doi.org/10.1101/2024.09.25.614879 New Results

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MOSAIC: a highly efficient, one-step recombineering approach to plasmid editing and diversification
Marijn van den Brink, Timotheus Y. Althuis, Christophe Danelon, Nico J. Claassens

bioRxiv 2024.03.22.586135; doi: https://doi.org/10.1101/2024.03.22.586135 New Results

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A yeast-based reverse genetics system to generate HCoV-OC43 reporter viruses encoding an eighth sgRNA
Brett A. Duguay, Trinity H. Tooley, Eric S. Pringle, John R. Rohde, Craig McCormick

bioRxiv 2024.09.23.614401; doi: https://doi.org/10.1101/2024.09.23.614401 New Results

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Rapid and reversible regulation of cell cycle progression in budding yeast using optogenetics
Andriana Koutsoumpa, Andreas Milias-Argeitis

bioRxiv 2024.09.21.614242; doi: https://doi.org/10.1101/2024.09.21.614242 New Results

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CRISPR-Cas-based Microhomology-Mediated End Joining for Precise Gene Replacement in Plant
Tien Van Vu, Gah-Hyun Lim, Seung Hee Choi, Ju Yeon Moon, Ngan Thi Nguyen, Swati Das, Mil Thi Tran, Yeon Woo Sung, Jihae Kim, Young Jong Song, Suk Weon Kim, Jae Cheol Jeong, Jae-Yean Kim

bioRxiv 2022.08.27.505510; doi: https://doi.org/10.1101/2022.08.27.505510 New Results

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Energy Aware Technology Mapping of Genetic Logic Circuits
Erik Kubaczka, Maximilian Gehri, Jérémie J. M. Marlhens, Tobias Schwarz, Maik Molderings, Nicolai Engelmann, Hernan G. Garcia, Christian Hochberger, Heinz Koeppl

bioRxiv 2024.06.27.601038; doi: https://doi.org/10.1101/2024.06.27.601038 New Results

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Synthetic Biology

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