Synthetic Biology

Controlling CRISPR-Cas9 with ligand-activated and ligand-deactivated sgRNAs
Kale Kundert, James E Lucas, Kyle E Watters, Christof Fellmann, Andrew H Ng, Benjamin M Heineike, Christina M Fitzsimmons, Benjamin L Oakes, David F Savage, Hana El-Samad, Jennifer A Doudna, Tanja Kortemme

bioRxiv 323105; doi: https://doi.org/10.1101/323105 New Results
Optimization of Golden Gate assembly through application of ligation sequence-dependent fidelity and bias profiling
Potapov Vladimir, Jennifer L. Ong, Rebecca B. Kucera, Bradley W. Langhorst, Katharina Bilotti, John M. Pryor, Eric J. Cantor, Barry Canton, Thomas F. Knight, Thomas C. Evans Jr., Gregory Lohman

bioRxiv 322297; doi: https://doi.org/10.1101/322297 New Results
Expression of novel fusion antiviral proteins Ricin A Chain-Pokeweed Antiviral Proteins (RTA-PAPs) in Escherichia coli and their inhibition of protein synthesis and of hepatitis B virus in vitro
Yasser Hassan, Sherry Ogg, Hui Ge

bioRxiv 322040; doi: https://doi.org/10.1101/322040 New Results
Filamentation and restoration of normal growth in E.coli using a combined CRISPRi sgRNA/antisense RNA approach
Andrea Mückl, Matthaeus Schwarz-Schilling, Katrin Fischer, Friedrich C Simmel

bioRxiv 323212; doi: https://doi.org/10.1101/323212 New Results
Long-distance communication in synthetic bacterial consortia through active signal propagation
James M Parkin, Richard M Murray

bioRxiv 321307; doi: https://doi.org/10.1101/321307 New Results
The Genetic Insulator RiboJ Increases Expression of Insulated Genes
Kalen P Clifton, Ethan M Jones, Sudip Paudel, John P Marken, Callan E Monette, Andrew D Halleran, Lidia Epp, Margaret S Saha

bioRxiv 317875; doi: https://doi.org/10.1101/317875 New Results
Enhancing the translational capacity of E. coli by resolving the codon bias
Zsuzsanna Gyorfy, Zoltan Lipinszki, Viktor Vernyik, Nora Farago, Tobias Sari, Laszlo Geza Puskas, Frederick Blattner, György Pósfai

bioRxiv 318105; doi: https://doi.org/10.1101/318105 New Results
Engineering Nitrogen Fixation Activity in an Oxygenic Phototroph
Deng Liu, Michelle Liberton, Jingjie Yu, Himadri Pakrasi, Maitrayee Bhattacharyya-Pakrasi

bioRxiv 316547; doi: https://doi.org/10.1101/316547 New Results
Genetic incompatibility combined with female-lethality is effective and robust in simulations of Aedes aegypti population control
Maciej Maselko, Stephen C Heinsch, Siba Das, Michael J Smanski

bioRxiv 316406; doi: https://doi.org/10.1101/316406 New Results
Coiled Coil-Mediated Assembly of an Icosahedral Protein Cage with Extremely High Thermal and Chemical Stability
Ajitha S Cristie-David, Junjie Chen, Derek B Nowak, Sung I Park, Mark M Banaszak Holl, Min Su, Neil Marsh

bioRxiv 316331; doi: https://doi.org/10.1101/316331 New Results

Synthetic Biology

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