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Design and implementation of a synthetic biomolecular concentration tracker

Victoria Hsiao, Emmanuel LC de los Santos, Weston R. Whitaker, John E. Dueber, Richard M. Murray
doi: https://doi.org/10.1101/000448
Victoria Hsiao
1Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, USA
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  • For correspondence: vhsiao@caltech.edu
Emmanuel LC de los Santos
1Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, USA
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Weston R. Whitaker
2Department of Microbiology and Immunology, Stanford University, Palo Alto, CA, USA
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John E. Dueber
3Department of Bioengineering, University of California, Berkeley, CA, USA
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Richard M. Murray
1Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, USA
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Abstract

As a field, synthetic biology strives to engineer increasingly complex artificial systems in living cells. Active feedback in closed loop systems offers a dynamic and adaptive way to ensure constant relative activity independent of intrinsic and extrinsic noise. In this work, we design, model, and implement a biomolecular concentration tracker, in which an output protein tracks the concentration of an input protein. Using synthetic scaffolds built from small, modular protein-protein interaction domains to colocalize a two-component system, the circuit design relies on a single negative feedback loop to modulate the production of the output protein. Using a combination of modeling and experimental work, we show that the circuit achieves real-time protein concentration tracking in Escherichia coli and that steady state outputs can be tuned.

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Posted December 10, 2013.
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Design and implementation of a synthetic biomolecular concentration tracker
Victoria Hsiao, Emmanuel LC de los Santos, Weston R. Whitaker, John E. Dueber, Richard M. Murray
bioRxiv 000448; doi: https://doi.org/10.1101/000448
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Design and implementation of a synthetic biomolecular concentration tracker
Victoria Hsiao, Emmanuel LC de los Santos, Weston R. Whitaker, John E. Dueber, Richard M. Murray
bioRxiv 000448; doi: https://doi.org/10.1101/000448

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