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PyRAD: assembly of de novo RADseq loci for phylogenetic analyses

Deren A. R. Eaton
doi: https://doi.org/10.1101/001081
Deren A. R. Eaton
1Committee on Evolutionary Biology, University of Chicago, 1025 E. 57th st. Chicago, IL 60637
2Botany Department, Field Museum of Natural History, 1400 S. Lake Shore Dr. Chicago, IL 60605
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Abstract

Restriction-site associated genomic markers are a powerful tool for investigating evolutionary questions at the population level, but are limited in their utility at deeper phylogenetic scales where fewer orthologous loci are typically recovered across disparate taxa. While this limitation stems in part from mutations to restriction recognition sites that disrupt data generation, an alternative source of data loss comes from the failure to identify homology during bioinformatic analyses. Clustering methods that allow for lower similarity thresholds and the inclusion of indel variation will perform better at assembling RADseq loci at the phylogenetic scale.

PyRAD is a pipeline to assemble de novo RADseq loci with the aim of optimizing coverage across phylogenetic data sets. It utilizes a wrapper around an alignment-clustering algorithm which allows for indel variation within and between samples, as well as for incomplete overlap among reads (e.g., paired-end). Here I compare PyRAD with the program Stacks in their performance analyzing a simulated RADseq data set that includes indel variation. Indels disrupt clustering of homologous loci in Stacks but not in PyRAD, such that the latter recovers more shared loci across disparate taxa. I show through re-analysis of an empirical RADseq data set that indels are a common feature of such data, even at shallow phylogenetic scales. PyRAD utilizes parallel processing as well as an optional hierarchical clustering method which allow it to rapidly assemble phylogenetic data sets with hundreds of sampled individuals.

Availability Software is written in Python and freely available at http://www.dereneaton.com/software/

Supplement Scripts to completely reproduce all simulated and empirical analyses are available in the Supplementary Materials.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY Unported 3.0 license.
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Posted December 03, 2013.
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PyRAD: assembly of de novo RADseq loci for phylogenetic analyses
Deren A. R. Eaton
bioRxiv 001081; doi: https://doi.org/10.1101/001081
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PyRAD: assembly of de novo RADseq loci for phylogenetic analyses
Deren A. R. Eaton
bioRxiv 001081; doi: https://doi.org/10.1101/001081

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