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Complementation of a temperature sensitive Escherichia coli rpoD mutation using Lactobacillus sigma factors

James D. Winkler, Katy C. Kao
doi: https://doi.org/10.1101/003152
James D. Winkler
*Department of Chemical Engineering, Texas A&M University, College Station, Texas, USA
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Katy C. Kao
†Department of Chemical Engineering, Texas A&M University, College Station, Texas, USA
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Abstract

Housekeeping sigma factors in the σ70 family, as components of the RNA polymerase holoenzyme, are responsible for regulating transcription of genes related to vegetative growth. While these factors are well understood in model organisms such as Escherichia coli and Bacillus subtilis, little experimental work has focused on the sigma factors in members of the Lactobacillus genus such as Lactobacillus brevis and Lactobacillus plantarum. This study evaluates the ability of putative σ70 proteins from L. brevis (Embedded Image) and L. plantarum (Embedded Image) to complement a temperature sensitive mutation in the E. coli 285c σ70 protein. This report is the first to show that these heterologous sigma factors were capable of restoring the viability of E. coli 285c for growth at 40-43.5 °C, indicating the Embedded Image and Embedded Image are capable of initiating transcription in a complex with the E. coli 285c RNA polymerase. These heterologous sigma factors may therefore be useful for improving biochemical knowledge of the sigma factor family or for use in the expression of hetereologous genomic libraries.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC 4.0 International license.
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Posted March 02, 2014.
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Complementation of a temperature sensitive Escherichia coli rpoD mutation using Lactobacillus sigma factors
James D. Winkler, Katy C. Kao
bioRxiv 003152; doi: https://doi.org/10.1101/003152
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Complementation of a temperature sensitive Escherichia coli rpoD mutation using Lactobacillus sigma factors
James D. Winkler, Katy C. Kao
bioRxiv 003152; doi: https://doi.org/10.1101/003152

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