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In vivo generation of DNA sequence diversity for cellular barcoding

Ian D. Peikon, Diana I. Gizatullina, Anthony M. Zador
doi: https://doi.org/10.1101/004499
Ian D. Peikon
1Watson School of Biological Sciences, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, 11724, USA
2Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, 11724, USA
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Diana I. Gizatullina
2Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, 11724, USA
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Anthony M. Zador
1Watson School of Biological Sciences, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, 11724, USA
2Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, 11724, USA
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  • For correspondence: zador@cshl.edu
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ABSTRACT

Heterogeneity is a ubiquitous feature of biological systems. A complete understanding of such systems requires a method for uniquely identifying and tracking individual components and their interactions with each other. We have developed a novel method of uniquely tagging individual cells in vivo with a genetic “barcode” that can be recovered by DNA sequencing. We demonstrate the feasibility of this technique in bacterial cells. This method should prove useful in tracking interactions of cells within a network, and/or heterogeneity within complex biological samples.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC 4.0 International license.
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Posted April 24, 2014.
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In vivo generation of DNA sequence diversity for cellular barcoding
Ian D. Peikon, Diana I. Gizatullina, Anthony M. Zador
bioRxiv 004499; doi: https://doi.org/10.1101/004499
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In vivo generation of DNA sequence diversity for cellular barcoding
Ian D. Peikon, Diana I. Gizatullina, Anthony M. Zador
bioRxiv 004499; doi: https://doi.org/10.1101/004499

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