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Generation of Aggregates of Mouse ES Cells that Show Symmetry Breaking, Polarisation and Emergent Collective Behaviour in vitro

Peter Baillie-Johnson, Susanne C. van den Brink, Tina Balayo, David A. Turner, Alfonso Martinez Arias
doi: https://doi.org/10.1101/005215
Peter Baillie-Johnson
Department of Genetics, University of Cambridge, Downing Street, Cambridge, CB2 3EH
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Susanne C. van den Brink
Department of Genetics, University of Cambridge, Downing Street, Cambridge, CB2 3EH
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Tina Balayo
Department of Genetics, University of Cambridge, Downing Street, Cambridge, CB2 3EH
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David A. Turner
Department of Genetics, University of Cambridge, Downing Street, Cambridge, CB2 3EH
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Alfonso Martinez Arias
Department of Genetics, University of Cambridge, Downing Street, Cambridge, CB2 3EH
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  • For correspondence: ama11@hermes.cam.ac.uk
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Abstract

Dissociated mouse embryonic stem (ES) cells were cultured to form aggregates in small volumes of basal medium in U-bottomed, non tissue-culture-treated 96-well plates and subsequently maintained in suspension culture. After growth for 48 hours, the aggregates are competent to respond to ubiquitous experimental signals which result in their symmetry-breaking and generation of defined polarised structures by 96 hours. It is envisaged that this system can be applied both to the study of early developmental events and more broadly to the processes of self-organisation and cellular decision-making. It may also provide a suitable niche for the generation of cell types present in the embryo but unobtainable from conventional adherent culture.

  • Abbreviations

    Chi
    CHIR99021
    DMH1
    Dorsomorphin-H1
    ES cell
    Embryonic Stem Cell
    PD03
    PD0325901
    SB43
    SB431542
  • Copyright 
    The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
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    Posted May 19, 2014.
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    Generation of Aggregates of Mouse ES Cells that Show Symmetry Breaking, Polarisation and Emergent Collective Behaviour in vitro
    Peter Baillie-Johnson, Susanne C. van den Brink, Tina Balayo, David A. Turner, Alfonso Martinez Arias
    bioRxiv 005215; doi: https://doi.org/10.1101/005215
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    Generation of Aggregates of Mouse ES Cells that Show Symmetry Breaking, Polarisation and Emergent Collective Behaviour in vitro
    Peter Baillie-Johnson, Susanne C. van den Brink, Tina Balayo, David A. Turner, Alfonso Martinez Arias
    bioRxiv 005215; doi: https://doi.org/10.1101/005215

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