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The genetic basis of energy conservation in the sulfate-reducing bacterium Desulfovibrio alaskensis G20

Morgan N. Price, Jayashree Ray, Kelly M. Wetmore, Jennifer V. Kuehl, Stefan Bauer, Adam M. Deutschbauer, Adam P. Arkin
doi: https://doi.org/10.1101/005694
Morgan N. Price
1Physical Biosciences Division, Lawrence Berkeley Lab, Berkeley, CA, USA
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Jayashree Ray
1Physical Biosciences Division, Lawrence Berkeley Lab, Berkeley, CA, USA
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Kelly M. Wetmore
1Physical Biosciences Division, Lawrence Berkeley Lab, Berkeley, CA, USA
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Jennifer V. Kuehl
1Physical Biosciences Division, Lawrence Berkeley Lab, Berkeley, CA, USA
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Stefan Bauer
2Energy Biosciences Institute, University of California, Berkeley, CA, USA
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Adam M. Deutschbauer
1Physical Biosciences Division, Lawrence Berkeley Lab, Berkeley, CA, USA
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Adam P. Arkin
1Physical Biosciences Division, Lawrence Berkeley Lab, Berkeley, CA, USA
2Energy Biosciences Institute, University of California, Berkeley, CA, USA
3Department of Bioengineering, University of California, Berkeley, CA, USA
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Abstract

Sulfate-reducing bacteria play major roles in the global carbon and sulfur cycles, but it remains unclear how reducing sulfate yields energy. To determine the genetic basis of energy conservation, we measured the fitness of thousands of pooled mutants of Desulfovibrio alaskensis G20 during growth in 12 different combinations of electron donors and acceptors. We show that ion pumping by the ferredoxin:NADH oxidoreductase Rnf is required whenever substrate-level phosphorylation is not possible. The uncharacterized complex Hdr/flox-1 (Dde_1207:13) is sometimes important alongside Rnf and may perform an electron bifurcation to generate more reduced ferredoxin from NADH to allow further ion pumping. Similarly, during the oxidation of malate or fumarate, the electron-bifurcating transhydrogenase NfnAB-2 (Dde_1250:1) is important and may generate reduced ferredoxin to allow additional ion pumping by Rnf. During formate oxidation, the periplasmic [NiFeSe] hydrogenase HysAB is required, which suggests that hydrogen forms in the periplasm, diffuses to the cytoplasm, and is used to reduce ferredoxin, thus providing a substrate for Rnf. During hydrogen utilization, the transmembrane electron transport complex Tmc is important and may move electrons from the periplasm into the cytoplasmic sulfite reduction pathway. Finally, mutants of many other putative electron carriers have no clear phenotype, which suggests that they are not important under our growth conditions.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
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Posted May 31, 2014.
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The genetic basis of energy conservation in the sulfate-reducing bacterium Desulfovibrio alaskensis G20
Morgan N. Price, Jayashree Ray, Kelly M. Wetmore, Jennifer V. Kuehl, Stefan Bauer, Adam M. Deutschbauer, Adam P. Arkin
bioRxiv 005694; doi: https://doi.org/10.1101/005694
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The genetic basis of energy conservation in the sulfate-reducing bacterium Desulfovibrio alaskensis G20
Morgan N. Price, Jayashree Ray, Kelly M. Wetmore, Jennifer V. Kuehl, Stefan Bauer, Adam M. Deutschbauer, Adam P. Arkin
bioRxiv 005694; doi: https://doi.org/10.1101/005694

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