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Natural variation in teosinte at the domestication locus teosinte branched1 (tb1)

Laura Vann, Thomas Kono, Tanja Pyhäjärvi, Matthew B. Hufford, Jeffrey Ross-Ibarra
doi: https://doi.org/10.1101/005819
Laura Vann
1Department of Plant Sciences, University of California Davis
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Thomas Kono
1Department of Plant Sciences, University of California Davis
2Department of Agronomy and Plant Genetics, University of Minnesota Twin Cities
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Tanja Pyhäjärvi
1Department of Plant Sciences, University of California Davis
3Department of Biology, University of Oulu
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Matthew B. Hufford
1Department of Plant Sciences, University of California Davis
4Department of Ecology, Evolution, and Organismal Biology, Iowa State University
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Jeffrey Ross-Ibarra
1Department of Plant Sciences, University of California Davis
5Center for Population Biology and Genome Center, University of California Davis
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Abstract

Premise of the study The teosinte branched1 (tb1) gene is a major QTL controlling branching differences between maize and its wild progenitor, teosinte. The insertion of a transposable element (Hopscotch) upstream of tb1 is known to enhance the gene’s expression, causing reduced tillering in maize. Observations of the maize tb1 allele in teosinte and estimates of an insertion age of the Hopscotch that predates domestication led us to investigate its prevalence and potential role in teosinte.

Methods Prevalence of the Hopscotch element was assessed across an Americas-wide sample of 837 maize and teosinte individuals using a co-dominant PCR assay. Population genetic summaries were calculated for a subset of individuals from four teosinte populations in central Mexico. Phenotypic data were also collected using seed from a single teosinte population where Hopscotch was found segregating at high frequency.

Key results Genotyping results indicate the Hopscotch element is found in a number of teosinte populations and linkage disequilibrium near tb1 does not support recent introgression from maize. Population genetic signatures are consistent with selection on this locus revealing a potential ecological role for Hopscotch in teosinte, but a greenhouse experiment does not detect a strong association between tb1 and tillering in teosinte.

Conclusions Our findings suggest the role of Hopscotch differs between maize and teosinte. Future work should assess tb1 expression levels in teosinte with and without the Hopscotch and more comprehensively phenotype teosinte to assess the ecological significance of the Hopscotch insertion and, more broadly, the tb1 locus in teosinte.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
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Posted September 11, 2014.
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Natural variation in teosinte at the domestication locus teosinte branched1 (tb1)
Laura Vann, Thomas Kono, Tanja Pyhäjärvi, Matthew B. Hufford, Jeffrey Ross-Ibarra
bioRxiv 005819; doi: https://doi.org/10.1101/005819
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Natural variation in teosinte at the domestication locus teosinte branched1 (tb1)
Laura Vann, Thomas Kono, Tanja Pyhäjärvi, Matthew B. Hufford, Jeffrey Ross-Ibarra
bioRxiv 005819; doi: https://doi.org/10.1101/005819

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