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Combining genome-scale Drosophila 3D neuroanatomical data by bridging template brains

View ORCID ProfileJames D. Manton, Aaron D. Ostrovsky, Lea Goetz, Marta Costa, Torsten Rohlfing, View ORCID ProfileGregory S. X. E. Jefferis
doi: https://doi.org/10.1101/006353
James D. Manton
1Division of Neurobiology, MRC Laboratory of Molecular Biology, Cambridge, CB2 0QH, UK
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Aaron D. Ostrovsky
1Division of Neurobiology, MRC Laboratory of Molecular Biology, Cambridge, CB2 0QH, UK
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Lea Goetz
1Division of Neurobiology, MRC Laboratory of Molecular Biology, Cambridge, CB2 0QH, UK
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Marta Costa
1Division of Neurobiology, MRC Laboratory of Molecular Biology, Cambridge, CB2 0QH, UK
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Torsten Rohlfing
2SRI International, Neuroscience Program, Center for Health Sciences, Menlo Park, CA, USA
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Gregory S. X. E. Jefferis
1Division of Neurobiology, MRC Laboratory of Molecular Biology, Cambridge, CB2 0QH, UK
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  • ORCID record for Gregory S. X. E. Jefferis
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Abstract

The stereotyped structure of mammalian and invertebrate brains is a crucial determinant of their circuit organization. Thus large scale efforts to map circuit organization using 3D image data are underway in a number of model systems, including flies and mice. Many of these studies use registration of sample images to a standard template brain to enable co-visualization and spatial querying. However, studies often use distinct template brains, resulting in large islands of data which cannot be directly compared. To enable this comparison, we have constructed bridging registrations between template brains accounting for the vast majority of Drosophila melanogaster 3D neuroanatomical data. Furthermore, we solve the related problem of mapping data between the left and right brain hemispheres via the construction of mirroring registrations. Finally, we extend our approach across species to demonstrate its potential use in evolutionary studies of neural circuit structure and provide bridging registrations that link a new set of template brains generated for four Drosophila species that are divergent over 40 million years of evolution.

We describe our strategy, document the freely available anatomical data and open source computer tools that we have generated and provide numerous examples of their use. This effort has unified data from over 30,000 publicly available images, with resources including the 3D atlas embodying the new standard Drosophila anatomical nomenclature and the largest single neuron databank yet available in any species. Over 20,000 registered images have been contributed to the Virtual Fly Brain project and can be viewed online at www.virtualflybrain.org.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted June 19, 2014.
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Combining genome-scale Drosophila 3D neuroanatomical data by bridging template brains
James D. Manton, Aaron D. Ostrovsky, Lea Goetz, Marta Costa, Torsten Rohlfing, Gregory S. X. E. Jefferis
bioRxiv 006353; doi: https://doi.org/10.1101/006353
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Combining genome-scale Drosophila 3D neuroanatomical data by bridging template brains
James D. Manton, Aaron D. Ostrovsky, Lea Goetz, Marta Costa, Torsten Rohlfing, Gregory S. X. E. Jefferis
bioRxiv 006353; doi: https://doi.org/10.1101/006353

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