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Efficient site-specific editing of the C. elegans genome

Donglei Zhang, Michael Glotzer
doi: https://doi.org/10.1101/007344
Donglei Zhang
Department of Molecular Genetics and Cell Biology, University of Chicago, CLSC 901, 920 E. 58th St., Chicago, IL 60637
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Michael Glotzer
Department of Molecular Genetics and Cell Biology, University of Chicago, CLSC 901, 920 E. 58th St., Chicago, IL 60637
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ABSTRACT

In just two years, genome editing with the CRISPR-associated endonuclease Cas9 has transformed genetic analysis in conventional and emerging model organisms. The efficiency of this method varies among systems and continues to be optimized. Numerous strategies have been reported for editing the C. elegans genome. To date, these strategies do not provide a simple, rapid and inexpensive means to introduce and isolate arbitrary point mutants. Here, we report a strategy with all three of these desirable properties. It utilizes oligonucleotides as donor templates for homology-dependent repair and visible markers that are edited in parallel that markedly reduce the number of animals that must be molecularly screened in order to isolate mutants that lack visible phenotypes.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted July 31, 2014.
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Efficient site-specific editing of the C. elegans genome
Donglei Zhang, Michael Glotzer
bioRxiv 007344; doi: https://doi.org/10.1101/007344
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Efficient site-specific editing of the C. elegans genome
Donglei Zhang, Michael Glotzer
bioRxiv 007344; doi: https://doi.org/10.1101/007344

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