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TACC3–ch-TOG track the growing tips of microtubules independently of clathrin and Aurora-A phosphorylation

Cristina Gutiérrez-Caballero, Selena G. Burgess, Richard Bayliss, View ORCID ProfileStephen J. Royle
doi: https://doi.org/10.1101/008359
Cristina Gutiérrez-Caballero
1Division of Biomedical Cell Biology, Warwick Medical School, Gibbet Hill Road, Coventry, CV4 7AL, UK
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Selena G. Burgess
2Department of Biochemistry, University of Leicester, Leicester LE1 9HN, UK
3Cancer Research UK Leicester Centre, UK
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Richard Bayliss
2Department of Biochemistry, University of Leicester, Leicester LE1 9HN, UK
3Cancer Research UK Leicester Centre, UK
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Stephen J. Royle
1Division of Biomedical Cell Biology, Warwick Medical School, Gibbet Hill Road, Coventry, CV4 7AL, UK
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  • ORCID record for Stephen J. Royle
  • For correspondence: s.j.royle@warwick.ac.uk
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Abstract

The interaction between TACC3 (transforming acidic coiled coil protein 3) and the microtubule polymerase ch-TOG (colonic, hepatic tumor overexpressed gene) is evolutionarily conserved. Loading of TACC3–ch-TOG onto spindle microtubules requires the phosphorylation of TACC3 by Aurora-A kinase and the subsequent interaction of TACC3 with clathrin to form a microtubule binding surface. Whether there is a pool of TACC3–ch-TOG that is independent of clathrin in human cells, and what is the function of this pool, are open questions. Here, we report that TACC3 is recruited to the plus-ends of microtubules by its association with ch-TOG and that this pool is independent of phosphorylation and binding to clathrin. The plus-end binding of TACC3–ch-TOG persists in interphase and we propose that one cellular function of TACC3–ch-TOG is to modulate cell migration. We also describe the distinct subcellular pools of TACC3, ch-TOG and clathrin. TACC3 is often described as a centrosomal protein, but we show that there is no significant population of TACC3 at centrosomes. The delineation of distinct protein pools reveals a simplified view of how these proteins are organized and controlled by post-translational modification.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
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Posted August 21, 2014.
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TACC3–ch-TOG track the growing tips of microtubules independently of clathrin and Aurora-A phosphorylation
Cristina Gutiérrez-Caballero, Selena G. Burgess, Richard Bayliss, Stephen J. Royle
bioRxiv 008359; doi: https://doi.org/10.1101/008359
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TACC3–ch-TOG track the growing tips of microtubules independently of clathrin and Aurora-A phosphorylation
Cristina Gutiérrez-Caballero, Selena G. Burgess, Richard Bayliss, Stephen J. Royle
bioRxiv 008359; doi: https://doi.org/10.1101/008359

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