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Systematic imaging reveals features and changing localization of mRNAs in Drosophila development

View ORCID ProfileHelena Jambor, View ORCID ProfileSurendranath Vineeth, View ORCID ProfileAlex T. Kalinka, View ORCID ProfilePavel Mejstrik, View ORCID ProfileStephan Saalfeld, View ORCID ProfilePavel Tomancak
doi: https://doi.org/10.1101/008938
Helena Jambor
1Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.
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  • For correspondence: jambor@mpicbg.de tomancak@mpi-cbg.de
Surendranath Vineeth
1Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.
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Alex T. Kalinka
1Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.
2Institute of Population Genetics Department of Biomedical Sciences, University of Veterinary Medicine Vienna, Austria.
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Pavel Mejstrik
1Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.
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Stephan Saalfeld
1Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.
3Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA.
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Pavel Tomancak
1Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.
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  • For correspondence: jambor@mpicbg.de tomancak@mpi-cbg.de
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Abstract

mRNA localization is critical for eukaryotic cells and affects numerous transcripts, yet how cells regulate distribution of many mRNAs to their subcellular destinations is still unknown. We combined transcriptomics and systematic imaging to determine tissue-specific expression and subcellular distribution of 5862 mRNAs during Drosophila oogenesis. mRNA localization is widespread in the ovary, depends on the microtubule cytoskeleton and the mRNAs enriched at the posterior cortex share common localization machinery. Localized mRNAs, in particular the posterior class, have distinct gene features and differ in expression level, 3’UTR length and sequence conservation from unlocalized mRNAs. Using cross-tissue comparison we revealed that the localization status of mRNAs differs between epithelial, germline and embryonic cell types and also changes within one cell, the oocyte, over time. This dataset enables the transition from deep mechanistic dissection of singular mRNA localization events towards global understanding of how mRNAs transcribed in the nucleus distribute in cells.

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Posted November 04, 2014.
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Systematic imaging reveals features and changing localization of mRNAs in Drosophila development
Helena Jambor, Surendranath Vineeth, Alex T. Kalinka, Pavel Mejstrik, Stephan Saalfeld, Pavel Tomancak
bioRxiv 008938; doi: https://doi.org/10.1101/008938
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Systematic imaging reveals features and changing localization of mRNAs in Drosophila development
Helena Jambor, Surendranath Vineeth, Alex T. Kalinka, Pavel Mejstrik, Stephan Saalfeld, Pavel Tomancak
bioRxiv 008938; doi: https://doi.org/10.1101/008938

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