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Characterization of the transcriptome, nucleotide sequence polymorphism, and natural selection in the desert adapted mouse Peromyscus eremicus

View ORCID ProfileMatthew D. MacManes, View ORCID ProfileMichael B. Eisen
doi: https://doi.org/10.1101/009134
Matthew D. MacManes
Department of Molecular, Cellular and Biomedical Sciences, University of New Hampshire, Durham NH, USA
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Michael B. Eisen
HHMI and University of California, Berkeley, Berkeley, CA, USA
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Abstract

As a direct result of intense heat and aridity, deserts are thought to be among the most harsh of environments, particularly for their mammalian inhabitants. Given that osmoregulation can be challenging for these animals, with failure resulting in death, strong selection should be observed on genes related to the maintenance of water and solute balance. One such animal, Peromyscus eremicus, is native to the desert regions of the southwest United States and may live its entire life without oral fluid intake. As a first step toward understanding the genetics that underlie this phenotype, we present a characterization of the P. eremicus transcriptome. We assay four tissues (kidney, liver, brain, testes) from a single individual and supplement this with population level renal transcriptome sequencing from 15 additional animals. We identified a set of transcripts undergoing both purifying and balancing selection based on estimates of Tajima’s D. In addition, we used the branch-site test to identify a transcript – Slc2a9, likely related to desert osmoregulation – undergoing enhanced selection in P. eremicus relative to a set of related non-desert rodents.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
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Posted September 29, 2014.
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Characterization of the transcriptome, nucleotide sequence polymorphism, and natural selection in the desert adapted mouse Peromyscus eremicus
Matthew D. MacManes, Michael B. Eisen
bioRxiv 009134; doi: https://doi.org/10.1101/009134
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Characterization of the transcriptome, nucleotide sequence polymorphism, and natural selection in the desert adapted mouse Peromyscus eremicus
Matthew D. MacManes, Michael B. Eisen
bioRxiv 009134; doi: https://doi.org/10.1101/009134

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