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Automated placement of stereotactic injections using a laser scan of the skull

Margaret Henderson, Vadim Pinskiy, Alexander Tolpygo, Stephen Savoia, Pascal Grange, Partha Mitra
doi: https://doi.org/10.1101/010603
Margaret Henderson
Cold Spring Harbor Laboratory
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Vadim Pinskiy
Cold Spring Harbor Laboratory
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Alexander Tolpygo
Cold Spring Harbor Laboratory
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Stephen Savoia
Cold Spring Harbor Laboratory
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Pascal Grange
Cold Spring Harbor Laboratory
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Partha Mitra
Cold Spring Harbor Laboratory
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Abstract

Stereotactic targeting is a commonly used technique for performing injections in the brains of mice and other animals. The most common method for targeting stereoscopic injections uses the skull indentations bregma and lambda as reference points and is limited in its precision by factors such as skull curvature and individual variation, as well as an incomplete correspondence between skull landmarks and brain locations. In this software tool, a 3D laser scan of the mouse skull is taken in vitro and registered onto a reference skull using a point cloud matching algorithm, and the parameters of the transformation are used to position a glass pipette to place tracer injections. The software was capable of registering sample skulls with less than 100 micron error, and was able to target an injection in a mouse with error of roughly 500 microns. These results indicate that using skull scan registration has the potential to be widely applicable in automating stereotactic targeting of tracer injections.

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Posted October 22, 2014.
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Automated placement of stereotactic injections using a laser scan of the skull
Margaret Henderson, Vadim Pinskiy, Alexander Tolpygo, Stephen Savoia, Pascal Grange, Partha Mitra
bioRxiv 010603; doi: https://doi.org/10.1101/010603
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Automated placement of stereotactic injections using a laser scan of the skull
Margaret Henderson, Vadim Pinskiy, Alexander Tolpygo, Stephen Savoia, Pascal Grange, Partha Mitra
bioRxiv 010603; doi: https://doi.org/10.1101/010603

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