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Resolving microbial microdiversity with high accuracy, full length 16S rRNA Illumina sequencing

Catherine M. Burke, Aaron E. Darling
doi: https://doi.org/10.1101/010967
Catherine M. Burke
1The ithree institute, University of Technology Sydney, NSW, Australia
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  • For correspondence: catherine.burke@uts.edu.au
Aaron E. Darling
1The ithree institute, University of Technology Sydney, NSW, Australia
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  • For correspondence: aaron.darling@uts.edu.au
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ABSTRACT

We describe a method for sequencing full-length 16S rRNA gene amplicons using the high throughput Illumina MiSeq platform. The resulting sequences have about 100-fold higher accuracy than standard Illumina reads and are chimera filtered using information from a single molecule dual tagging scheme that boosts the signal available for chimera detection. We demonstrate that the data provides fine scale phylogenetic resolution not available from Illumina amplicon methods targeting smaller variable regions of the 16S rRNA gene.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
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Posted November 06, 2014.
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Resolving microbial microdiversity with high accuracy, full length 16S rRNA Illumina sequencing
Catherine M. Burke, Aaron E. Darling
bioRxiv 010967; doi: https://doi.org/10.1101/010967
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Resolving microbial microdiversity with high accuracy, full length 16S rRNA Illumina sequencing
Catherine M. Burke, Aaron E. Darling
bioRxiv 010967; doi: https://doi.org/10.1101/010967

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