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The role of TORC1 in muscle development in Drosophila

Isabelle Hatfield, Innocence Harvey, Erika. R. Yates, JeAnna R. Redd, Lawrence T. Reiter, View ORCID ProfileDave Bridges
doi: https://doi.org/10.1101/010991
Isabelle Hatfield
*Department of Physiology, University of Tennessee Health Science Center
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Innocence Harvey
*Department of Physiology, University of Tennessee Health Science Center
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Erika. R. Yates
*Department of Physiology, University of Tennessee Health Science Center
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JeAnna R. Redd
*Department of Physiology, University of Tennessee Health Science Center
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Lawrence T. Reiter
†Department of Neurology, University of Tennessee Health Science Center
‡Department of Pediatrics, University of Tennessee Health Science Center and Children’s Foundation Research Institute, Le Bonheur Children’s Hospital
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Dave Bridges
*Department of Physiology, University of Tennessee Health Science Center
‡Department of Pediatrics, University of Tennessee Health Science Center and Children’s Foundation Research Institute, Le Bonheur Children’s Hospital
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  • ORCID record for Dave Bridges
  • For correspondence: dbridge9@uthsc.edu
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Abstract

Myogenesis is an important process during both development and muscle repair. Previous studies suggest that mTORC1 plays a role in the formation of mature muscle from immature muscle precursor cells. Here we show that gene expression for several myogenic transcription factors including Myf5, Myog and Mef2c but not MyoD and myosin heavy chain isoforms decrease when C2C12 cells are treated with rapamycin, supporting a role for mTORC1 pathway during muscle development. To investigate the possibility that mTORC1 can regulate muscle in vivo we ablated the essential dTORC1 subunit Raptor in Drosophila melanogaster and found that muscle-specific knockdown of Raptor causes flies to be too weak to emerge from their pupal cases during eclosion. Using a series of GAL4 drivers we also show that muscle-specific Raptor knockdown also causes shortened lifespan, even when eclosure is unaffected. Together these results highlight an important role for TORC1 in muscle development, integrity and function in both Drosophila and mammalian cells.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
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Posted December 23, 2014.
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The role of TORC1 in muscle development in Drosophila
Isabelle Hatfield, Innocence Harvey, Erika. R. Yates, JeAnna R. Redd, Lawrence T. Reiter, Dave Bridges
bioRxiv 010991; doi: https://doi.org/10.1101/010991
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The role of TORC1 in muscle development in Drosophila
Isabelle Hatfield, Innocence Harvey, Erika. R. Yates, JeAnna R. Redd, Lawrence T. Reiter, Dave Bridges
bioRxiv 010991; doi: https://doi.org/10.1101/010991

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