Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

GLO-Roots: an imaging platform enabling multidimensional characterization of soil-grown roots systems

View ORCID ProfileRubén Rellán-Álvarez, View ORCID ProfileGuillaume Lobet, View ORCID ProfileHeike Lindner, View ORCID ProfilePierre-Luc Pradier, View ORCID ProfileJose Sebastian, View ORCID ProfileMuh-Ching Yee, View ORCID ProfileYu Geng, View ORCID ProfileCharlotte Trontin, View ORCID ProfileTherese LaRue, View ORCID ProfileAmanda Schrager Lavelle, View ORCID ProfileCara H. Haney, View ORCID ProfileRita Nieu, View ORCID ProfileJulin Maloof, View ORCID ProfileJohn P. Vogel, View ORCID ProfileJosé R. Dinneny
doi: https://doi.org/10.1101/016931
Rubén Rellán-Álvarez
1Department of Plant Biology, Carnegie Institution for Science, Stanford, CA, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Rubén Rellán-Álvarez
Guillaume Lobet
2PhytoSystems, University of Liège, Liège, Belgium
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Guillaume Lobet
Heike Lindner
1Department of Plant Biology, Carnegie Institution for Science, Stanford, CA, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Heike Lindner
Pierre-Luc Pradier
1Department of Plant Biology, Carnegie Institution for Science, Stanford, CA, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Pierre-Luc Pradier
Jose Sebastian
1Department of Plant Biology, Carnegie Institution for Science, Stanford, CA, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Jose Sebastian
Muh-Ching Yee
1Department of Plant Biology, Carnegie Institution for Science, Stanford, CA, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Muh-Ching Yee
Yu Geng
1Department of Plant Biology, Carnegie Institution for Science, Stanford, CA, USA
7DOE Joint Genome Institute, Walnut Creek, CA, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Yu Geng
Charlotte Trontin
1Department of Plant Biology, Carnegie Institution for Science, Stanford, CA, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Charlotte Trontin
Therese LaRue
3Department of Biology, Stanford University, Stanford, CA, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Therese LaRue
Amanda Schrager Lavelle
4Department of Plant Biology, UC Davis, Davis, CA, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Amanda Schrager Lavelle
Cara H. Haney
5Harvard Medical School, Massachusetts General Hospital, Department of Genetics, DEpartment of Molecular Biology, Boston, MA, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Cara H. Haney
Rita Nieu
6USDA Western Regional Research Center, Albany, CA, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Rita Nieu
Julin Maloof
4Department of Plant Biology, UC Davis, Davis, CA, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Julin Maloof
John P. Vogel
7DOE Joint Genome Institute, Walnut Creek, CA, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for John P. Vogel
José R. Dinneny
1Department of Plant Biology, Carnegie Institution for Science, Stanford, CA, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for José R. Dinneny
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

Abstract

Root systems develop different root types that individually sense cues from their local environment and integrate this information with systemic signals. This complex multidimensional amalgam of inputs enables continuous adjustment of root growth rates, direction and metabolic activity that define a dynamic physical network. Current methods for analyzing root biology balance physiological relevance with imaging capability. To bridge this divide, we developed an integrated imaging system called Growth and Luminescence Observatory for Roots (GLO-Roots) that uses luminescence-based reporters to enable studies of root architecture and gene expression patterns in soil-grown, light-shielded roots. We have developed image analysis algorithms that allow the spatial integration of soil properties, gene expression and root system architecture traits. We propose GLO-Roots as a system that has great utility in presenting environmental stimuli to roots in ways that evoke natural adaptive responses and in providing tools for studying the multi-dimensional nature of such processes.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
Back to top
PreviousNext
Posted August 03, 2015.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
GLO-Roots: an imaging platform enabling multidimensional characterization of soil-grown roots systems
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
GLO-Roots: an imaging platform enabling multidimensional characterization of soil-grown roots systems
Rubén Rellán-Álvarez, Guillaume Lobet, Heike Lindner, Pierre-Luc Pradier, Jose Sebastian, Muh-Ching Yee, Yu Geng, Charlotte Trontin, Therese LaRue, Amanda Schrager Lavelle, Cara H. Haney, Rita Nieu, Julin Maloof, John P. Vogel, José R. Dinneny
bioRxiv 016931; doi: https://doi.org/10.1101/016931
Reddit logo Twitter logo Facebook logo LinkedIn logo Mendeley logo
Citation Tools
GLO-Roots: an imaging platform enabling multidimensional characterization of soil-grown roots systems
Rubén Rellán-Álvarez, Guillaume Lobet, Heike Lindner, Pierre-Luc Pradier, Jose Sebastian, Muh-Ching Yee, Yu Geng, Charlotte Trontin, Therese LaRue, Amanda Schrager Lavelle, Cara H. Haney, Rita Nieu, Julin Maloof, John P. Vogel, José R. Dinneny
bioRxiv 016931; doi: https://doi.org/10.1101/016931

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Plant Biology
Subject Areas
All Articles
  • Animal Behavior and Cognition (4384)
  • Biochemistry (9609)
  • Bioengineering (7103)
  • Bioinformatics (24896)
  • Biophysics (12632)
  • Cancer Biology (9974)
  • Cell Biology (14372)
  • Clinical Trials (138)
  • Developmental Biology (7966)
  • Ecology (12124)
  • Epidemiology (2067)
  • Evolutionary Biology (16002)
  • Genetics (10936)
  • Genomics (14755)
  • Immunology (9880)
  • Microbiology (23697)
  • Molecular Biology (9490)
  • Neuroscience (50924)
  • Paleontology (370)
  • Pathology (1541)
  • Pharmacology and Toxicology (2686)
  • Physiology (4023)
  • Plant Biology (8674)
  • Scientific Communication and Education (1511)
  • Synthetic Biology (2402)
  • Systems Biology (6444)
  • Zoology (1346)